GSTT1 null genotype is a risk factor for diabetic retinopathy in Caucasians with type 2 diabetes, whereas GSTM1 null genotype might confer protection against retinopathy
GSTT1 null genotype is a risk factor for diabetic retinopathy in Caucasians with type 2 diabetes, whereas GSTM1 null genotype might confer protection against retinopathy
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DOI：
10.1155/2012/675628
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发表时间：
2012-01-01
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影响因子：
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通讯作者：
Petrovic, Daniel
中科院分区：
文献类型：
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作者：
Cilensek, Ines;Mankoc, Sara;Petrovic, Daniel
目的：大量数据表明氧化应激参与糖尿病视网膜病变（DR）的发展。本研究的目的是研究遗传多态性：谷胱甘肽s -转移酶M1 （GSTM1）和T1 （GSTT1）的多态性缺失以及GSTP1的Ile105Val是否与斯洛文尼亚2型糖尿病患者的DR相关。方法：采用横断面病例-对照研究方法，选取604例无血缘关系的斯洛文尼亚（高加索）2型糖尿病患者，其中284例为DR（例），320例为无DR临床症状的10年以上2型糖尿病患者作为对照组，采用聚合酶链反应（PCR）和限制性内切片段长度多态性（RFLP）测定基因型。结果：在我们的研究中，DR患者中GSTM1基因缺失的频率低于对照组（27.5%比44.4%,P < 0.001），而GSTT1基因缺失的频率明显高于对照组（49.3%比29.7%,P < 0.001）。我们没有发现GSTP1 （Ile105Val）多态性基因型分布在病例和对照组之间的统计学差异（40.5%对46.0%）。结论：我们可以得出结论，GSTT1纯合缺失的个体发生DR的风险大约是2倍，而GSTM1缺乏与2型糖尿病患者发生DR的频率较低有关。
Aim: Substantial data indicate that oxidative stress is involved in the development of diabetic retinopathy (DR). The aim of the present study was to investigate whether the genetic polymorphisms: polymorphic deletions of glutathione S-transferases M1 (GSTM1) and T1 (GSTT1) and Ile105Val of the GSTP1 are associated with DR in Slovenian patients with type 2 diabetes.Methods: In this cross sectional case-control study 604 unrelated Slovene subjects (Caucasians) with type 2 diabetes mellitus were enrolled: 284 patients with DR (cases) and the control group of 320 subjects with type 2 diabetes of more than 10 years' duration who had no clinical signs of DR. Genotypes were determined by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP).Results: In our study, the deletion of the GSTM1 was found less frequent in cases with DR than in the controls (27.5% versus 44.4%; P < 0.001), whereas the deletion of GSTT1 was found significantly more often in cases than in the controls (49.3% versus 29.7%; P < 0.001). We did not find statistically significant differences in the genotype distribution in GSTP1 (Ile105Val) polymorphism between cases and controls (40.5% versus 46.0%).Conclusions: We may conclude that individuals homozygous for the deletion of GSTT1 are at an approximate to 2-fold-greater risk of DR, whereas the GSTM1 deficiency is associated with lower frequency of DR in type 2 diabetics.