Convergent sensing pathways mediate response to two extracellular competence factors in Bacillus subtilis.
Convergent sensing pathways mediate response to two extracellular competence factors in Bacillus subtilis.
复制标题
收敛传感途径介导对枯草芽孢杆菌中两种细胞外能力因子的反应。
DOI：
10.1101/gad.9.5.547
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发表时间：
1995
影响因子：
10.5
通讯作者：
Grossman,AD
中科院分区：
文献类型：
--
作者：
Solomon,JM;Magnuson,R;Srivastava,A;Grossman,AD
枯草芽孢杆菌中遗传能力的发展受细胞外信号分子调节，包括ComX信息素，一种修饰的9或10氨基酸肽。在这里，我们提出的第二个细胞外感受态刺激因子（CSF）的表征。CSF似乎至少部分是520和720道尔顿之间的小肽。CSF的产生需要几个基因，这些基因对于孢子形成的起始和感受态的发育都是必需的（spo0H、spo0A、spo0B和spo0F）。虽然这两种肽因子调节能力，两种不同的传感途径介导的ComX信息素和CSF的响应。双突变体的分析表明，ComX信息素是在相同的遗传途径的膜结合组氨酸蛋白激酶编码的comP和CSF是在相同的遗传途径的寡肽通透酶编码的spo0K。此外，部分纯化的ComX信息素的细胞反应需要ComP组氨酸蛋白激酶，而部分纯化的CSF的反应需要Spo0K寡肽通透酶。这两条感应通路汇聚在一起激活感受态基因。这两个因素和他们的会聚传感通路所需的正常发展的能力，并可能发挥作用，以整合不同的生理信号。
Development of genetic competence in Bacillus subtilis is regulated by extracellular signaling molecules, including the ComX pheromone, a modified 9- or 10-amino-acid peptide. Here, we present characterization of a second extracellular competence stimulating factor (CSF). CSF appears to be, at least in part, a small peptide of between 520 and 720 daltons. Production of CSF requires several genes that are needed both for initiation of sporulation and development of competence (spo0H, spo0A, spo0B, and spo0F). Although both peptide factors regulate competence, two different sensing pathways mediate the response to the ComX pheromone and CSF. Analysis of double mutants indicated that ComX pheromone is on the same genetic pathway as the membrane-bound histidine protein kinase encoded by comP and that CSF is on the same genetic pathway as the oligopeptide permease encoded by spo0K. Furthermore, the cellular response to partly purified ComX pheromone requires the ComP histidine protein kinase, whereas the response to partly purified CSF requires the Spo0K oligopeptide permease. These two sensing pathways converge to activate competence genes. Both factors and their convergent sensing pathways are required for normal development of competence and might function to integrate different physiological signals.