A novel electrochemical DNA biosensor based on graphene and polyaniline nanowires
A novel electrochemical DNA biosensor based on graphene and polyaniline nanowires
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DOI：
10.1016/j.electacta.2010.12.034
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发表时间：
2011-02-15
影响因子：
6.6
通讯作者：
Huang, Shasheng
中科院分区：
文献类型：
--
作者：
Bo, Yang;Yang, Huiyan;Huang, Shasheng
以氧化石墨烯和聚苯胺纳米线（PANIws）修饰玻碳电极，制备了一种新型的DNA生物传感器。所得石墨烯/PANlw层对互补DNA序列表现出良好的DPV电流响应。石墨烯和PANIw的作用可能是其良好的电子转移活性的原因。采用扫描电子显微镜（SEM）、循环伏安法（CV）和电化学阻抗谱（EIS）对石墨烯和聚苯胺纳米膜进行了表征。由于石墨烯和聚苯胺的独特协同效应，大大提高了探针DNA在电极表面的固定化。在最佳条件下，该传感器对DNA具有快速的安培响应，高灵敏度和良好的储存稳定性。在2.12 × 10 ~（-6）~ 2.12 × 10 ~（-12）mol·l ~（-1）范围内，传感器的电流响应与靶浓度呈线性关系，相关系数为0.9938。检测限（3 σ）为3.25 × 10（-13）mol l（-1）。结果表明，该修饰电极在灵敏、选择性DNA检测方面具有潜在的应用价值。(C)2010爱思唯尔有限公司版权所有。
A novel DNA biosensor based on oxidized graphene and polyaniline nanowires (PANIws) modified glassy carbon electrode was developed. The resulting graphene/PANlw layers exhibited good DPV current response for the complementary DNA sequences. The good electron transfer activity might be attributed to the effect of graphene and PANIw. Graphene and PANIw nanolayers film with highly conductive and biocompatible nanostructure were characterized by scanning electron microscopy (SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The immobilization of the probe DNA on the surface of electrode was largely improved due to the unique synergetic effect of graphene and PANIw. Under optimum conditions, the biosensor exhibited a fast amperometric response, high sensitivity and good storage stability for monitoring DNA. The current response of the sensor increases linearly with the concentration of target from 2.12 x 10(-6) to 2.12 x 10(-12) mol l(-1) with a relative coefficient of 0.9938. The detection limit (3 sigma) is 3.25 x 10(-13) mol l(-1). The results indicate that this modified electrode has potential application in sensitive and selective DNA detection. (C) 2010 Elsevier Ltd. All rights reserved.