In vivo real-time, multicolor, quantum dot lymphatic imaging.
In vivo real-time, multicolor, quantum dot lymphatic imaging.
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DOI：
10.1038/jid.2009.161
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发表时间：
2009-12
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淋巴网络复杂且难以在体内实时可视化。此外，淋巴网络内的流动方向通常是不可预测的，尤其是在“分水岭”发达或淋巴管重叠的区域。在此，我们报告了一种使用镉-硒量子点（Qdots）和荧光成像系统进行体内实时多色淋巴成像的方法，该系统能够同时实时可视化多达五个不同的淋巴盆地。选择五个视觉上可很好区分的羧基 Qdot（Qdot 545、565、585、605 和 655），并在五个不同部位皮下注射到小鼠体内，并在手术中通过实时多色淋巴成像进行体内或原位连续成像。在所有七只小鼠中，体内淋巴图像成功地实时区分了所有五个具有不同颜色的淋巴盆地。这些淋巴结可视化持续至少 7 天。这种方法在淋巴研究中具有相当大的潜力，用于研究淋巴系统内的解剖结构和流动，以及在一些有限的临床环境中，实时可见荧光可以促进手术或内窥镜检查下的手术。
The lymphatic network is complex and difficult to visualize in real-time in vivo. Moreover, the direction of flow within lymphatic networks is often unpredictable especially in areas with well-developed “watershed” or overlapping lymphatics. Herein, we report a method of in vivo real-time multicolor lymphatic imaging using cadmium–selenium quantum dots (Qdots) with a fluorescence imaging system that enables the simultaneous visualization of up to five distinct lymphatic basins in real-time. Five visually well-distinguishable carboxyl-Qdots (Qdot 545, 565, 585, 605, and 655) were selected and injected subdermally into mice at five different sites, and serially imaged in vivo or in situ under surgery with real-time multicolor lymphatic imaging. In all seven mice, in vivo lymphatic images successfully distinguished all five lymphatic basins with different colors in realtime. These visualizations of lymph node lasted up to at least 7 days. This method could have a considerable potential in lymphatic research for studying the anatomy and flow within the lymphatic system as well as in some limited clinical settings where real-time visible fluorescence could facilitate procedures under surgery or endoscopy.