D-amino acid oxidase-nanoparticle system: a potential novel approach for cancer enzymatic therapy
D-amino acid oxidase-nanoparticle system: a potential novel approach for cancer enzymatic therapy
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DOI：
10.2217/nnm.12.187
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发表时间：
2013-11-01
期刊：
影响因子：
5.5
通讯作者：
Bernardini, Giovanni
中科院分区：
文献类型：
--
作者：
Bava, Adriana;Gornati, Rosalba;Bernardini, Giovanni
目的：作者提出了一种新的磁性纳米颗粒-酶系统用于癌症治疗，能够靶向酶，从而减少不良反应，同时改善患者的生活质量。材料和方法：作者用3-氨基丙基三乙氧基硅烷（APTES）官能化Fe 3 O 4纳米颗粒，并通过与戊二醛偶联将其缀合至酵母D-氨基酸氧化酶（DAAO）。结果与结论：作者在三种肿瘤细胞系上测试了Fe 3 O 4-APTES-DAAO系统。在电子显微镜水平上，暴露的细胞显示出质膜表面和内吞囊泡内部的纳米颗粒。Fe_3 O_4-APTES-DAAO引起细胞活力的显著下降，当加入DAAO底物D-丙氨酸时，细胞活力大大增强。Fe 3 O 4-APTES-DAAO被证明比游离DAAO更有效，证实了该系统在癌症治疗中的有效性。原文提交日期2012年3月27日;修订版提交日期2012年9月20日;在线发布日期2013年2月5日
Aim: The authors propose a new magnetic nanoparticle-enzyme system for cancer therapy capable of targeting the enzyme and consequently decreasing the adverse effects, meanwhile improving the patient's life quality. Materials & methods: The authors have functionalized Fe3O4 nanoparticles with 3-amino-propyltriethoxysilane (APTES) and conjugated it to yeast D-amino acid oxidase (DAAO) by coupling this with glutaraldehyde. Results & conclusion: The authors have tested the Fe3O4-APTES-DAAO system on three tumor cell lines. Exposed cells show, at the electron microscope level, nanoparticles on the surface of the plasma membrane and inside endocytic vesicles. Fe3O4-APTES-DAAO caused a substantial decrease of cell viability greatly augmented when D-alanine, a DAAO substrate, was added. Fe3O4-APTES-DAAO was demonstrated to be more effective than free DAAO, confirming the validity of the system in cancer therapy. Original submitted 27 March 2012; Revised submitted 20 September 2012; Published online 5 February 2013