Comprehensive Monosynaptic Rabies Virus Mapping of Host Connectivity with Neural Progenitor Grafts after Spinal Cord Injury.
Comprehensive Monosynaptic Rabies Virus Mapping of Host Connectivity with Neural Progenitor Grafts after Spinal Cord Injury.
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DOI：
10.1016/j.stemcr.2017.04.004
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发表时间：
2017-06-06
影响因子：
5.9
通讯作者：
Tuszynski MH
中科院分区：
文献类型：
--
作者：
Adler AF;Lee-Kubli C;Kumamaru H;Kadoya K;Tuszynski MH
Neural progenitor cells grafted to sites of spinal cord injury have supported electrophysiological and functional recovery in several studies. Mechanisms associated with graft-related improvements in outcome appear dependent on functional synaptic integration of graft and host systems, although the extent and diversity of synaptic integration of grafts with hosts are unknown. Using transgenic mouse spinal neural progenitor cell grafts expressing the TVA and G-protein components of the modified rabies virus system, we initiated monosynaptic tracing strictly from graft neurons placed in sites of cervical spinal cord injury. We find that graft neurons receive synaptic inputs from virtually every known host system that normally innervates the spinal cord, including numerous cortical, brainstem, spinal cord, and dorsal root ganglia inputs. Thus, implanted neural progenitor cells receive an extensive range of host neural inputs to the injury site, potentially enabling functional restoration across multiple systems. Transgenic embryos expressed modified rabies virus system components TVA/G-protein Transgenic neural grafts initiated monosynaptic rabies tracing after cervical SCI All major known spinal projections formed new synaptic contacts onto grafts Neural progenitor cell grafts may be capable of restoring a diversity of functions In this article, Tuszynski and colleagues demonstrate that embryonic spinal neural progenitor cells grafted to sites of spinal cord injury can accept synaptic contacts from all major known host systems that project to the intact spinal cord. To achieve this, they initiated monosynaptic rabies virus tracing from transgenic spinal neural progenitor cell grafts expressing TVA and G-protein.
