Spatio-temporal analysis of mitotic activity with a labile cyclin-GUS fusion protein
Spatio-temporal analysis of mitotic activity with a labile cyclin-GUS fusion protein
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DOI：
10.1046/j.1365-313x.1999.00620.x
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发表时间：
1999-11-01
期刊：
影响因子：
7.2
通讯作者：
Doerner, P
中科院分区：
文献类型：
--
作者：
Colón-Carmona, A;You, R;Doerner, P
植物生长对发育和环境信号反应迅速，但对细胞分裂活动的潜在变化知之甚少。开发了一种不稳定的细胞周期蛋白-GUS报告基因，以促进细胞分裂模式的时空分析。嵌合报告蛋白在每个细胞周期中翻转，因此其组织化学活性准确地报告单个有丝分裂细胞。使用拟南芥植物转化与细胞周期蛋白-GUS，我们可视化模式的有丝分裂活动在受伤的叶片，这表明细胞分裂的作用，在结构加固。
Plant growth responds rapidly to developmental and environmental signals, but the underlying changes in cell division activity are poorly understood. A labile cyclin-GUS reporter was developed to facilitate the spatio-temporal analysis of cell division patterns. The chimeric reporter protein is turned over every cell cycle and hence its histochemical activity accurately reports individual mitotic cells. Using Arabidopsis plants transformed with cyclin-GUS, we visualized patterns of mitotic activity in wounded leaves which suggest a role for cell division in structural reinforcement.