Discrimination Analysis of Blood Plasma Associated with Alzheimer's Disease Using Vibrational Spectroscopy
Discrimination Analysis of Blood Plasma Associated with Alzheimer's Disease Using Vibrational Spectroscopy
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DOI：
10.3233/jad-122041
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发表时间：
2013-01-01
影响因子：
4
通讯作者：
Toledanog, Adolfo
中科院分区：
文献类型：
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作者：
Carmona, Pedro;Molina, Marina;Toledanog, Adolfo
在这项研究中，我们已经确定是否拉曼和红外光谱的血浆区分阿尔茨海默病（AD）从正常老化的健康对照。采用傅里叶变换光谱仪和近红外激光束作为拉曼光谱的激发源，对8名轻度AD、16名中度AD、11名重度AD和12名正常老年对照者的血浆样品进行光谱分析。光谱进行处理，采用判别分析，以确定是否频带面积和频率-强度关系可能揭示与AD相关的生化差异。在1700-1600 cm（-1）的拉曼光谱范围内鉴定出7种光谱生物标志物（蛋白质二级结构），980-910 cm（-1）（蛋白质α-螺旋），790-730 cm（-1）（蛋白质三级结构），和440-390 cm（-1）（蛋白质骨架）以及1700-1600 cm（-1）（蛋白质二级结构）和1150-1000 cm（-1）（氧化应激）的红外区域。该判别分析模型区分AD与正常老年对照组的敏感性为89%，特异性为92%。此外，检测轻度AD的特异性增加至100%。总之，我们的研究结果打开了使用这种光谱方法作为早期准确诊断AD的非侵入性，快速和相对便宜的程序的可能性。
In this study we have determined whether Raman and infrared spectroscopy of blood plasma differentiates Alzheimer's disease (AD) from normal aging of healthy controls. Spectroscopic analysis was conducted on blood plasma samples from 8 mild AD, 16 moderate AD, 11 severe AD, and 12 normal elderly control persons using Fourier transform spectrometers and a near-infrared laser beam as excitation source for Raman spectroscopy. Spectra were processed employing discriminant analysis to determine whether band areas and frequency-intensity relationships might reveal biochemical differences associated with AD. Seven spectral biomarkers were identified in the Raman regions of 1700-1600 cm(-1) (protein secondary structure), 980-910 cm(-1) (protein alpha-helices), 790-730 cm(-1) (protein tertiary structure), and 440-390 cm(-1) (protein backbone) and in the infrared regions of 1700-1600 cm(-1) (protein secondary structure) and 1150-1000 cm(-1) (oxidative stress). This discriminant analysis model differentiated AD from normal aging of elderly control persons with a sensitivity of 89% and specificity of 92%. Moreover, specificity increases to 100% for the detection of mild AD. In summary, our results open the possibility of using this spectroscopic approach as a non-invasive, rapid, and relatively inexpensive procedure for early accurate diagnosis of AD.