A murine monoclonal IgM antibody specific for blood group P antigen (globoside)
A murine monoclonal IgM antibody specific for blood group P antigen (globoside)
复制标题
针对 P 血型抗原（球苷）特异的鼠单克隆 IgM 抗体
DOI：
10.1111/j.1365-2141.1986.tb07492.x
复制
发表时间：
1986
影响因子：
6.5
通讯作者：
P. Tetteroo
中科院分区：
文献类型：
--
作者：
A. E. G. Kr. vondem Borne;M. J. Bos;N. Joustra;J. Tromp;R. van Wijngaarden;P. Tetteroo
鼠单克隆IgM红细胞抗体似乎具有抗P（抗球蛋白苷）特异性。该抗体是一种相对较弱的冷凝集素，但具有较强的溶血素，其与红细胞的反应性通过酶处理显著增强。该抗体用于研究球孢糖苷在细胞和组织中的分布。红细胞、成红细胞、内皮细胞、血小板亚群、巨核细胞和成纤维细胞均能检测到赤霉素。在粒细胞、单核细胞和大多数外周血淋巴细胞中未检测到。在成红细胞前体细胞（CFU-E、BFU-E）、原成红细胞或原成红细胞细胞系K562和HEL的细胞上也不存在。而K562细胞在丁酸钠诱导下向成红细胞成熟时表达球蛋白苷。还测试了各种白血病患者的细胞。在CML急变（CML‐BC）病例中经常观察到大量阳性反应细胞（18例中有6例），而在AML病例中很少观察到（37例中有4例）。在CML-BC中，P-阳性细胞可能是成红细胞和/或成巨核细胞。因此，红血球苷似乎是成红细胞型或混合成红细胞-巨核细胞型CML-BC的一个有趣的标志物。
A murine monoclonal IgM erythrocyte antibody appeared to have anti‐P (anti‐globoside) specificity. The antibody was a relatively weak cold agglutinin, but a strong haemolysin and its reactivity with red cells was markedly enhanced by enzyme treatment. This antibody was used to study the cell and tissue distribution of globoside. Globoside was not only detectable on red cells and erythroblasts, but also on endothelial cells and on subsets of platelets, megakaryocytes and fibroblasts. It was not detectable on granulocytes, monocytes and most peripheral blood lymphocytes. Neither was it present on erythroblast precursors (CFU‐E, BFU‐E), pro‐erythroblasts or on the cells of the pro‐erythroblastic cell lines K562 and HEL. However, K562 cells expressed globoside when induced to mature into erythroblasts by sodium butyrate. Cells of patients with various leukaemias were also tested. A significant number of positively reacting cells was frequently (six out of 18) seen in cases with a CML blast crisis (CML‐BC) and rarely in AML (four out of 37 cases). In CML‐BC the P‐positive cells were probably erythroblasts and/or megakaryoblasts. Thus, globoside appeared to be an interesting marker in CML‐BC of the erythroblastic or mixed erythroblastic‐megakaryoblastic type.
人红白血病细胞系 HEL 和 K562 的碳水化合物抗原谱。
影响因子：
20.3
作者：
Kannagi,R;Papayannopoulou,T;Nakamoto,B;Cochran,NA;Yokochi,T;Stamatoyannopoulos,G;Hakomori,S
通讯作者：
Hakomori,S
球苷和福斯曼糖脂在红细胞膜上的定位。
DOI：
10.1016/0005-2736(83)90086-x
发表时间：
1983
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Tillack,TW;Allietta,M;Moran,RE;YoungJr,WW
通讯作者：
YoungJr,WW