Development and characterization of microsatellite loci in Brasenia schreberi (Cabombaceae) based on the next-generation sequencing
Development and characterization of microsatellite loci in Brasenia schreberi (Cabombaceae) based on the next-generation sequencing
复制标题
基于新一代测序的莼菜（Cabombaceae）微卫星位点的开发和表征
DOI：
10.4238/gmr.15027886
复制
发表时间：
2016-01-01
影响因子：
0.4
通讯作者：
Liao, K.
中科院分区：
文献类型：
--
作者：
Liu, H.;Long, Z. C.;Liao, K.
为了了解濒危水生草本植物Brasenia schreberi （cabombacae）的遗传变异，我们描述了利用新一代测序获得的31个微卫星标记。对来自美国杰克逊湖种群的24个个体进行了每个标记的基因分型。28个标记是多态性的。每个位点的等位基因数为1 ~ 9个；观察到的杂合度为0 ~ 1，期望杂合度为0 ~ 0.751。这些标记可作为雪氏芽孢杆菌遗传变异和保护研究的有效工具。
To understand genetic variability of the endangered aquatic herb Brasenia schreberi (Cabombaceae), we describe 31 microsatellite markers obtained using next-generation sequencing. A total of 24 individuals from the population of Jackson Lake, USA, were genotyped for each marker. Twenty-eight markers were polymorphic. The number of alleles per locus ranged from 1 to 9; the observed and expected heterozygosities ranged from 0 to 1 and from 0 to 0.751, respectively. These markers should be useful tools for genetic variation and conservation studies of B. schreberi.