A nitric oxide concentration clamp.
A nitric oxide concentration clamp.
复制标题
DOI：
10.1006/niox.1999.0253
复制
发表时间：
1999-10
期刊：
影响因子：
--
通讯作者：
V. Zhelyaskov;D. Godwin
中科院分区：
文献类型：
--
作者：
V. Zhelyaskov;D. Godwin
我们报道了一种产生一氧化氮(NO)的新方法，该方法具有几个优点，可用于实验。该方法由一个光解室组成，在光解室中，用氙灯发出的光照射不发光的NO供体，并结合反馈控制来释放NO。通过在光发射到将光传送到光解室的光导中之前选择性地选通通过快门投射的光来实现对光解光的控制。通过按照传感器报告来自光解室的几乎瞬时浓度的比例来选通光，可以实现无浓度的标准，该标准可以很容易地调整到更高或更低的标准水平。为了表示这一过程与电压钳的电生理过程的相似性，我们将这一过程称为浓度“钳”。这一发展加强了基于光纤的系统对NO输送的使用，并应能够在NO的原位浓度特别关键的地方进行实验，例如在生物制剂中。
We report a new method of generating nitric oxide (NO) that possesses several advantages for experimental use. This method consists of a photolysis chamber where NO is released by illuminating photolabile NO donors with light from a xenon lamp, in conjunction with feedback control. Control of the photolysis light was achieved by selectively gating light projected through a shutter before the light was launched into a light guide that conveyed the light to the photolysis chamber. By gating the light in proportion to a sensor that reported nearly instantaneous concentration from the photolysis chamber, a criterion NO concentration could be achieved, which could be easily adjusted to higher or lower criterion levels. To denote the similarity of this process with the electrophysiological process of voltage clamp, we term this process a concentration "clamp." This development enhances the use of the fiber-optic-based system for NO delivery and should enable the execution of experiments where the in situ concentration of NO is particularly critical, such as in biological preparations.