The Emerging Role of Zfp217 in Adipogenesis.
The Emerging Role of Zfp217 in Adipogenesis.
复制标题
Zfp217 在脂肪形成中的新作用
DOI：
10.3390/ijms18071367
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发表时间：
2017-06-27
影响因子：
5.6
通讯作者：
Jiang SW
中科院分区：
文献类型：
--
作者：
Xiang H;Zhong ZX;Peng YD;Jiang SW
锌指蛋白 217 (Zfp217) 是 krüppel 型锌指蛋白家族的成员，在哺乳动物细胞分化和发育中发挥多种作用。尽管对 Zfp217 在癌症、多能性和重编程中的功能进行了广泛的研究，但其在脂肪生成中的生理作用仍然未知。我们之前的 RNA 测序数据表明 Zfp217 参与脂肪形成。本研究通过生物信息学分析和一系列实验研究了Zfp217在脂肪生成中的潜在功能。在C3H10T1/2细胞的成脂分化过程中发现Zfp217的表达逐渐上调，这与成脂标记基因Pparg2的表达一致。此外，Zfp217 表达与脂肪细胞分化之间存在显着的正相关关系。还观察到Zfp217不仅可以引发C3H10T1/2细胞的增殖缺陷，还可以与Ezh2相互作用并抑制Ezh2的下游靶基因。此外，三种靶向 Zfp217 的 microRNA（miR-503-5p、miR-135a-5p 和 miR-19a-3p）被发现可以抑制脂肪生成过程。这是第一份表明 Zfp217 具有调节脂肪生成能力的报告。
Zinc finger protein 217 (Zfp217), a member of the krüppel-type zinc finger protein family, plays diverse roles in cell differentiation and development of mammals. Despite extensive research on the functions of Zfp217 in cancer, pluripotency and reprogramming, its physiological roles in adipogenesis remain unknown. Our previous RNA sequencing data suggest the involvement of Zfp217 in adipogenesis. In this study, the potential function of Zfp217 in adipogenesis was investigated through bioinformatics analysis and a series of experiments. The expression of Zfp217 was found to be gradually upregulated during the adipogenic differentiation in C3H10T1/2 cells, which was consistent with that of the adipogenic marker gene Pparg2. Furthermore, there was a positive, significant relationship between Zfp217 expression and adipocyte differentiation. It was also observed that Zfp217 could not only trigger proliferative defect in C3H10T1/2 cells, but also interact with Ezh2 and suppress the downstream target genes of Ezh2. Besides, three microRNAs (miR-503-5p, miR-135a-5p and miR-19a-3p) which target Zfp217 were found to suppress the process of adipogenesis. This is the first report showing that Zfp217 has the capacity to regulate adipogenesis.
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DOI：
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发表时间：
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期刊：
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影响因子：
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DOI：
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发表时间：
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影响因子：
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