Production of protease from a new alkalophilic Bacillus sp I-312 grown on soybean meal:: optimization and some properties
Production of protease from a new alkalophilic Bacillus sp I-312 grown on soybean meal:: optimization and some properties
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DOI：
10.1016/j.procbio.2004.05.010
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发表时间：
2005-03-01
影响因子：
4.4
通讯作者：
Chang, CS
中科院分区：
文献类型：
--
作者：
Joo, HS;Chang, CS
一种具有工业意义的氧化性和SDS稳定性碱性蛋白酶，由嗜碱芽孢杆菌I-312产生，该菌株分离自韩国西海仁川松岛附近严重污染的潮泥滩和海水。当细菌在含有（g 1（-1））：大豆粉，15;小麦粉，10;果糖，5; K2 HPO 4，4; Na 2 HPO 4，1- CaCl，0.05; Na 2CO 3，8的培养基中在32 ℃下培养48小时，以250 rpm搅拌时，获得最大活性（41520 U ml（-1））。该酶的最适pH为1.5左右，最适温度为60 ℃。碱性蛋白酶对SDS和氧化剂也表现出极高的稳定性，用5%SDS和5%H2O2处理72 h，其活性分别保持87.8%和87.5%。芽孢杆菌I-312分泌的碱性丝氨酸蛋白酶除了对SDS和过氧化氢的稳定性之外，从其在碱性pH中起作用和在宽pH范围内显示稳定性的能力的角度来看，在工业上是重要的，这表明它是作为洗涤剂制剂中的添加剂的潜在候选物。(C)2004爱思唯尔有限公司保留所有权利。
An oxidative and SDS-stable alkaline protease having industrial significance and produced by an alkalophilic Bacillus sp. I-312, which was isolated from the heavily polluted tidal mud flat and sea water near Songdo in Inchon, Korean West Sea (Yellow Sea). Maximum activity (41520 U ml(-1)) was obtained when the bacterium was grown in a medium containing (g 1(-1)): soybean meal, 15; wheat flour, 10; fructose, 5; K2HPO4, 4: Na2HPO4, l - CaCl,, 0.05; Na2CO3, 8 at 32 degreesC for 48 It incubation period with agitation of 250 rpm. The optimum pH and temperature of the enzyme were around I I and 60 degreesC, respectively. The alkaline protease also showed extreme stability towards SDS and oxidizing agents, which retained its activity 87.8 and 87.5% activity on treatment with 5% SDS and 5% H2O2 for 72 h, respectively. The alkaline serine protease secreted by Bacillus sp. I-312 is industrially important from the perspectives of its abilities to function in alkaline pH and to show stability in broad pH ranges in addition to its stability towards SDS and hydrogen peroxide, suggesting that it is a potential candidate as an additive in detergent formulations. (C) 2004 Elsevier Ltd. All rights reserved.