SIMPLE AND RAPID MEASUREMENT OF HUMAN LYMPHOCYTES-T AND THEIR SUBCLASSES IN PERIPHERAL-BLOOD
SIMPLE AND RAPID MEASUREMENT OF HUMAN LYMPHOCYTES-T AND THEIR SUBCLASSES IN PERIPHERAL-BLOOD
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DOI：
10.1073/pnas.77.8.4914
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发表时间：
1980-01-01
期刊：
影响因子：
--
通讯作者：
GOLDSTEIN, G
中科院分区：
文献类型：
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作者：
HOFFMAN, RA;KUNG, PC;GOLDSTEIN, G
描述了一种简单快速的测定血沉棕黄层制剂或全血中人 T 淋巴细胞亚类的方法。该技术使用流式细胞术根据淋巴细胞的光散射特性将淋巴细胞与其他白细胞区分开。通过细胞免疫荧光计数淋巴细胞亚类；免疫荧光信号由针对 T 细胞表面分化抗原的单克隆抗体产生。计数 T 淋巴细胞亚类的传统技术需要耗时（长达 2 天）的密度梯度和红细胞玫瑰花结富集，并且需要至少 20 ml 的血液。这里描述的方法对于每种测试的抗体使用少至50μl的全血并在2小时内产生结果。
A simple and rapid method for the determination of human T-lymphocyte subclasses in buffy coat preparations or whole blood is described. This technique uses flow cytometry to distinguish lymphocytes from other leukocytes on the basis of their light-scattering properties. Lymphocyte subclasses were enumerated by cellular immunofluorescence; the immunofluorescent signals were produced by monoclonal antibodies to surface differentiation antigens on T cells. Conventional techniques of enumerating T-lymphocyte subclasses entail time-consuming (up to 2 days) density gradient and E[erythrocyte]-rosette enrichment, and require at least 20 ml of blood. The method described here uses as little as 50 .mu.l of whole blood for each antibody tested and produces results within 2 h.