Construction of small RNA cDNA libraries for deep sequencing
Construction of small RNA cDNA libraries for deep sequencing
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DOI：
10.1016/j.ymeth.2007.05.002
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发表时间：
2007-10-01
期刊：
影响因子：
4.8
通讯作者：
Green, Pamela J.
中科院分区：
文献类型：
--
作者：
Lu, Cheng;Meyers, Blake C.;Green, Pamela J.
小RNA（21-24个核苷酸）包括microRNA（miRNAs）和小干扰RNA（siRNAs）是植物和动物中基因表达的有效调节剂。已经通过克隆方法实验鉴定了数百个编码miRNA和数千个SiRNA的基因。新的测序技术促进了这些分子的鉴定，并提供了给定生物样品中的全局定量表达数据。在这里，我们描述了在我们的实验室中使用的方法来构建小RNA cDNA文库的高通量测序技术，如MPSS，454或SBS。(C)2007爱思唯尔公司All rights reserved.
Small RNAs (21-24 nucleotides) including microRNAs (miRNAs) and small interfering RNAs (siRNAs) are potent regulators of gene expression in both plants and animals. Several hundred genes encoding miRNAs and thousands of siRNAs have been experimentally identified by cloning approaches. New sequencing technologies facilitate the identification of these molecules and provide global quantitative expression data in a given biological sample. Here, we describe the methods used in our laboratory to construct small RNA cDNA libraries for high-throughput sequencing using technologies such as MPSS, 454 or SBS. (C) 2007 Elsevier Inc. All rights reserved.