Preharvest application of selenite enhances the quality of Chinese flowering cabbage during storage via regulating the ascorbate-glutathione cycle and phenylpropanoid metabolisms
Preharvest application of selenite enhances the quality of Chinese flowering cabbage during storage via regulating the ascorbate-glutathione cycle and phenylpropanoid metabolisms
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采前施用亚硒酸盐通过调节抗坏血酸-谷胱甘肽循环和苯丙烷代谢来提高大白菜贮藏期间的品质
DOI：
10.1016/j.foodres.2022.112229
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发表时间：
2022-11-28
影响因子：
8.1
通讯作者：
Wang, Guang
中科院分区：
文献类型：
--
作者：
Chen, Zhuosheng;Zhang, Ling;Wang, Guang
菜心（Brassica campestris L.）SSP.中国变种utilis Tsen et Lee）是一种潜在的硒富集植物，但采前硒处理是否以及如何影响采后品质尚不清楚。结果表明，采前根际施用100 μ mol/L的亚硒酸钠可提高菜心的贮藏品质。它增加了抗氧化能力，减少了收获后的重量损失、叶片黄化和蛋白质降解。提高了贮藏期间POD、CAT、GSH-Px、GR等抗氧化酶的活性以及阿萨、GSH、酚类物质和类黄酮的含量。代谢物组学分析表明，酚酸，包括p-香豆酸，咖啡酸，阿魏酸;黄酮类化合物，如柚皮素，圣草酚，芹菜素，槲皮素，山奈酚，及其衍生物显着增加采前亚硒酸盐处理。与对照相比，亚硒酸盐处理的甘蓝总抗氧化能力（DPPH法、ABTS法和FRAP法）均显著增强。转录组学分析表明，亚硒酸盐诱导的DEGs在AsA-GSH代谢和苯丙素类生物合成途径中显著富集。此外，采前亚硒酸盐处理显著上调了贮藏期间BrGST、BrGSH-Px、BrAPX、BrASO、BrC 4 H、BrCOMT、BrCHS和BrFLS的表达。这些结果表明，采前亚硒酸盐处理不仅通过增加硒的生物积累，而且还通过调节AsA-GSH循环和增加采后酚类和黄酮类化合物的合成来提高甘蓝的品质。本研究为探讨采前硒处理对菜心贮藏品质的影响提供了新的思路。
Chinese flowering cabbage (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee) is a candidate of selenium (Se) accumulator, but it is not clear whether and how preharvest Se treatment affects its quality after harvest. Here, we showed that preharvest application of 100 mu mol/L selenite to roots enhanced storage quality of Chinese flowering cabbage. It increased antioxidant capacity and reduced weight loss, leaf yellowing, and protein degradation after harvest. Furthermore, it increased the activities of antioxidant enzymes such as POD, CAT, GSH-Px, and GR, as well as contents of AsA, GSH, phenolics, and flavonoids during storage. Metabolome analysis revealed that phenolic acids including p-Coumaric acid, caffeic acid, and ferulic acid; flavonoids such as naringenin, eriodictyol, apigenin, quercetin, kaempferol, and their derivatives were notably increased by preharvest selenite treatment. Consistently, the total antioxidant capacity, evaluated by DPPH, ABTS, and FRAP methods, were all markedly enhanced in selenite-treated cabbage compared to the control. Transcriptomics analysis showed that the DEGs induced by selenite were significantly enriched in AsA-GSH metabolisms and phenylpropanoids biosynthesis pathways. Moreover, preharvest selenite treatment significantly up-regulated the expressions of BrGST, BrGSH-Px, BrAPX, BrASO, BrC4H, BrCOMT, BrCHS, and BrFLS during storage. These results suggest that preharvest selenite treatment enhanced quality of cabbage not only by increasing Se biological accumulation, but also through regulating AsA-GSH cycle and increasing phenolics and flavonoids synthesis after harvest. This study provides a novel insight into the effects of preharvest Se treatment on quality of Chinese flowering cabbage during storage.