OPTIMIZATION OF A QUANTITATIVE HPLC DETERMINATION OF POTENTIALLY ANTICARCINOGENIC FLAVONOIDS IN VEGETABLES AND FRUITS
OPTIMIZATION OF A QUANTITATIVE HPLC DETERMINATION OF POTENTIALLY ANTICARCINOGENIC FLAVONOIDS IN VEGETABLES AND FRUITS
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DOI：
10.1021/jf00021a023
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发表时间：
1992-09-01
影响因子：
6.1
通讯作者：
VENEMA, DP
中科院分区：
文献类型：
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作者：
HERTOG, MGL;HOLLMAN, PCH;VENEMA, DP
建立了一种基于反相高效液相色谱-紫外检测器的快速测定冻干蔬菜和水果中5种主要黄酮苷元的方法，即槲皮素、山奈酚、杨梅素、木犀草素和芹菜素。水解和提取的完整性已被优化，通过系统测试不同的条件，如酸浓度，反应时间，和甲醇浓度的提取溶液中使用的样品含有各种类型的黄酮苷。提出了黄酮醇葡萄糖醛酸苷、黄酮醇糖苷和黄酮苷的最佳水解条件。用二极管阵列确认黄酮类化合物的身份。方法重复性好，变异系数槲皮素为2.5- 3.1%，山奈酚为4.6- 5.6%，杨梅素为4.6%，木犀草素为3.3%，芹菜素为2.8%。实验室内重现性的CV小于重复性CV的2倍。黄酮醇类槲皮素，山奈酚，杨梅素的回收率范围从77至110%，和黄酮类芹菜素和木犀草素的回收率范围从99至106%。提出的方法允许一个快速，定量和重现性测定冻干食品中的五种黄酮类化合物。
A rapid method based on RP-HPLC with UV detection is presented for the quantitative determination of five major flavonoid aglycons, viz. quercetin, kaempferol, myricetin, luteolin, and apigenin, in freeze-dried vegetables and fruits, after acid hydrolysis of the parent glycosides. Completeness of hydrolysis and extraction have been optimized by testing systematically different conditions such as acid concentration, reaction period, and methanol concentration in the extraction solution using samples containing various types of flavonoid glycosides. Optimum hydrolysis conditions are presented for flavonol glucuronides, flavonol glucosides, and flavone glycosides. Identity of the flavonoids was confirmed with diode array. Repeatability of the method was good, with coefficients of variation of 2.5-3.1 % for quercetin, 4.6-5.6 % for kaempferol, 4.6 % for myricetin, 3.3 % for luteolin, and 2.8 % for apigenin. CV of the within-laboratory reproducibility was less than 2 times the CV of repeatability. Recoveries of the flavonols quercetin, kaempferol, and myricetin ranged from 77 to 110 %, and recoveries of the flavones apigenin and luteolin ranged from 99 to 106 %. The method presented allows a fast, quantitative, and reproducible determination of five flavonoids in freeze-dried foods.