Study on in situ analysis of cellulose, hemicelluloses and lignin distribution linked to tissue structure of crop stalk internodal transverse section based on FTIR microspectroscopic imaging
Study on in situ analysis of cellulose, hemicelluloses and lignin distribution linked to tissue structure of crop stalk internodal transverse section based on FTIR microspectroscopic imaging
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基于FTIR显微光谱成像的农作物秸秆节间横切面组织结构相关纤维素、半纤维素和木质素分布的原位分析研究
DOI：
10.1007/s10570-014-0525-7
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发表时间：
2015-02-01
期刊：
影响因子：
5.7
通讯作者：
Ji, Guanya
中科院分区：
文献类型：
--
作者：
Cao, Cong;Yang, Zengling;Ji, Guanya
农作物秸秆是木质纤维素生物质的重要来源，作为一种可再生资源已被广泛开发。与作物秸秆解剖学相关的组织成分异质性对植物研究人员和生物质精炼领域的研究人员很有吸引力。本研究旨在开发一种基于傅里叶变换红外（FTIR）显微光谱成像的高效、信息丰富的表征技术，以实现对农作物秸秆的深入了解，以玉米和棉花为例，创造性地从化学意义上“看到”农作物秸秆的组织结构，生成了可直观地与组织结构联系起来的纤维素、半纤维素和木质素的化学图像。 具有诊断光谱峰的 FTIR 显微光谱成像。然后应用主成分分析来揭示不同组织之间的主要光谱差异。对于棉秆横切面，木质素高度分布在木质部，而纤维素分布均匀，半纤维素高度分布在髓部，与木质素相反。玉米秆节间横切面，木质素浓缩 在机械组织中，纤维素分布相对均匀，而与木质素相反，半纤维素集中在实质细胞中。结果表明，FTIR 显微光谱成像是分析作物茎横切面组织结构相关化学特征的理想技术。
Crop stalks are an important source of lignocellulosic biomass, and have been broadly developed as one kind of renewable resources. Tissue compositional heterogeneity linked to crop stalks anatomy is attractive to plant researchers and researchers in the field of the biomass-refinery. This study is to develop a high-efficiency and rich-information characterization technique based on Fourier transform infrared (FTIR) microspectroscopic imaging for achieving an insight into the crop stalks, in which corn and cotton are taken for example, by creatively "seeing'' crop stalks tissue structure in a chemical sense. Chemical images of cellulose, hemicelluloses and lignin that can be intuitively linked to tissue structure were produced based on FTIR microspectroscopic imaging with diagnostic spectral peaks. The principal components analysis was then applied to reveal the major spectral variances among different tissues. For cotton stalk transverse section, lignin is highly distributed in the xylem, while cellulose is uniformly distributed, and hemicellulose is highly distributed in the pith, in contrast to lignin. For corn stalk internodal transverse section, lignin is concentrated in the mechanical tissues, and again, cellulose is relatively uniformly distributed, while hemicellulose is concentrated in the parenchymal cells, in contrast to lignin. The results show that FTIR microspectroscopic imaging is an ideal technique for analyzing the chemical characterization linked to tissue structure in crop stalk transverse section.