INFLUENCE OF MANGANESE ON GROWTH OF A SHEATHLESS STRAIN OF LEPTOTHRIX-DISCOPHORA
INFLUENCE OF MANGANESE ON GROWTH OF A SHEATHLESS STRAIN OF LEPTOTHRIX-DISCOPHORA
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DOI：
10.1128/aem.49.3.556-562.1985
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发表时间：
1985-01-01
影响因子：
4.4
通讯作者：
GHIORSE, WC
中科院分区：
文献类型：
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作者：
ADAMS, LF;GHIORSE, WC
Mn2+ 对分批培养中的 L. discophora 菌株 SS-1 的生长产生不同的影响，具体取决于添加到培养基中的浓度。与分离菌株SS-1的环境中的浓度相比，0.55-5.5μM Mn2+的浓度降低了细胞产量并延长了稳定期存活，但不影响生长速率。 55-910μM Mn2+浓度升高也降低了细胞产量并延长了存活时间，但生长速率也降低了。 1820μM Mn2+的添加导致细胞数量下降，随后在温育80小时后呈指数上升，表明细胞群体的发展对Mn2+毒性具有抵抗力。当将360μM或更少的Mn2+添加到生长烧瓶中时，Mn2+被氧化成氧化锰(MnOx，其中x约为2)，其在培养基中呈现为棕色颗粒。添加55μM Mn2+的培养物生长期间Mn氧化的定量表明，几乎所有的Mn2+在生长稳定期开始时(15-25小时)被氧化。这一结果表明，在低浓度和中等浓度的 Mn2+ 下观察到的细胞产量下降与 MnOx 的形成有关，MnOx 可能结合了 SS-1 生长必需的阳离子营养物质。向含有55μM Mn2+的培养物中添加过量的Fe3+使细胞产量增加至接近未添加Mn2+的培养物中发现的水平，这表明MnOx造成的Fe剥夺至少部分地导致了细胞产量的降低。
Mn2+ exerted various effects on the growth of L. discophora strain SS-1 in batch cultures depending on the concentration added to the medium. Concentrations of 0.55-5.5 .mu.M Mn2+, comparable to those in the environment from which strain SS-1 was isolated, decreased cell yield and prolonged stationary-phase survival, but did not affect growth rate. Elevated concentrations of 55-910 .mu.M Mn2+ also decreased cell yield and prolonged survival, but growth rate was decreased as well. The addition of 1820 .mu.M Mn2+ caused a decline in cell numbers followed by an exponential rise after 80 h of incubation, indicating the development of a population of cells resistant to Mn2+ toxicity. When 360 .mu.M Mn2+ or less was added to growth flasks, Mn2+ was oxidized to manganese oxide (MnOx, where x is .apprx. 2), which appeared as brown particles in the medium. Quantification of Mn oxidation during growth of cultures to which 55 .mu.M Mn2+ was added showed that nearly all of the Mn2+ was oxidized by the beginning of the stationary phase of growth (15-25 h). This result suggested that the decrease in cell yield observed at low and moderate concentrations of Mn2+ was related to the formation of MnOx, which may have bound cationic nutrients essential to the growth of SS-1. The addition of excess Fe3+ to cultures containing 55 .mu.M Mn2+ increased cell yield to levels near those found in cultures with no added Mn2+, indicating that Fe-deprivation by MnOx was at least partly responsible for the decreased cell yield.