Correlating fibronectin adsorption with endothelial cell adhesion and signaling on polymer substrates
Correlating fibronectin adsorption with endothelial cell adhesion and signaling on polymer substrates
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DOI：
10.1002/jbm.a.10316
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发表时间：
2003-01-01
影响因子：
4.9
通讯作者：
Grainger, DW
中科院分区：
文献类型：
--
作者：
Koenig, AL;Gambillara, V;Grainger, DW
研究了纤连蛋白（Fn）在不同商业聚合物表面化学上的吸附，包括组织培养聚苯乙烯（TCPS）、细菌学聚苯乙烯（BPS）、含氟聚合物Teflon AF（（R））和聚-L-丙交酯（PLLA）。抗体探针检测Fn的细胞结合结构域的可用性吸附Fn在竞争性存在和不存在的牛血清白蛋白（BSA）。结构域的可用性是最高的Fn吸附在TCPS上，特别是在血清白蛋白或稀释血清的存在下。在血清和无血清培养基中，在预先吸附Fn的表面上培养的人脐静脉内皮细胞（HUVEC）的附着和生长效率与抗体细胞结合结构域的可用性相关：TCPS> BPS，Teflon AF（（R））> PLLA。在Teflon AF（（R））表面上培养的细胞中，来自GTdR，RhoA的细胞内信号传导最高（RhoA：RhoGDI抑制剂比率），表明尽管与TCPS相比Teflon AF（（R））上附着的细胞数量较低，但细胞信号传导在生长24小时后仍然活化。使用RT-PCR技术评估的上调的细胞Fn mRNA信息支持HUVEC产生内源性细胞外基质（ECM）蛋白Fn，以便在次优的Teflon AF（（R））培养表面上附着和存活。(C)2002 Wiley Periodicals，Inc.
Fibronectin (Fn) adsorption was studied on different commercial polymer surface chemistries, including tissue culture polystyrene (TCPS), bacteriologic polystyrene (BPS), fluoropolymer Teflon AF((R)), and poly-L-lactide (PLLA). Antibody probes detected the availability of Fn's cell binding domain on adsorbed Fn in the competitive presence and absence of bovine serum albumin (BSA). Domain availability was highest for Fn adsorbed on TCPS, especially in the presence of either serum albumin or dilute serum. Attachment and growth efficiencies for human umbilical venous endothelial cells (HUVECs) cultured on surfaces preadsorbed with Fn in serum and serum-free media correlated with antibody cell-binding domain availability: TCPS > BPS, Teflon AF((R)) > PLLA. Intracellular signaling from the GTPase, RhoA, was highest (RhoA:RhoGDI inhibitor ratio) in cells cultured on the Teflon AF((R)) surfaces, indicating that despite lower attached cell numbers on Teflon AF((R)) compared to TCPS, cell signaling remained activated after 24 h of growth. Up-regulated cellular Fn mRNA messages, assessed using RT-PCR techniques, supported HUVECs' producing the endogenous extracellular matrix (ECM) protein Fn in order to attach and survive on the suboptimal Teflon AF((R)) culture surfaces. (C) 2002 Wiley Periodicals, Inc.