PHOSPHATIDYLCHOLINE PROTECTS AGAINST FIBROSIS AND CIRRHOSIS IN THE BABOON
PHOSPHATIDYLCHOLINE PROTECTS AGAINST FIBROSIS AND CIRRHOSIS IN THE BABOON
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DOI：
10.1016/s0016-5085(94)95023-7
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发表时间：
1994-01-01
期刊：
影响因子：
29.4
通讯作者：
LEO, MA
中科院分区：
文献类型：
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作者：
LIEBER, CS;ROBINS, SJ;LEO, MA
背景/目的多不饱和大豆卵磷脂（55%-60%磷脂酰胆碱[PC]）可防止酒精喂养狒狒的纤维化。本研究是为了确定PC是否是活性剂。（相当于卵磷脂中所含的）在有或没有酒精的情况下给药长达6.5年，并将结果与未补充组的结果进行比较。而12只狒狒中有10只在无PC的情况下喂食酒精，发生间隔纤维化或肝硬化，81% ± 3%的肝脂肪细胞转化为产生胶原的移行细胞。相比之下，8只喂食酒精和PC的动物中没有一只发生间隔纤维化或肝硬化，只有48% ± 9%的脂肪细胞转化，表明PC确实是保护性化合物。乙醇喂养也导致肝磷脂和PC减少，两者都被补充剂纠正。此外，PC刺激胶原酶活性在培养的脂肪细胞。该PC由几种物质组成，主要是二亚油酰磷脂酰胆碱（40%-52%）和棕榈酰-亚油酰磷脂酰胆碱（23%-24%）。只有二亚油酰磷脂酰胆碱复制PC对胶原酶的作用。其他物种的PC，磷脂酰乙醇胺，游离脂肪酸，或胆碱没有effect.ConclusionsPC防止酒精诱导的纤维化和肝硬化在非人灵长类动物，和二亚油酰磷脂酰胆碱似乎是活性物种，可能通过促进胶原蛋白分解。
Background/AimsPolyunsaturated soybean lecithin (55%–60% phosphatidylcholine [PC]) protects against fibrosis in alcohol-fed baboons. The present study was undertaken to determine whether PC is the active agent.MethodsVirtually pure PC (equivalent to that contained in the lecithin) was administered for up to 6.5 years with or without alcohol, and the results were compared with those of unsupplemented groups.ResultsControl livers remained normal, whereas 10 of 12 baboons fed alcohol without PC developed septal fibrosis or cirrhosis with transformation of 81% ± 3% of the hepatic lipocytes to collagen-producing transitional cells. By contrast, none of the eight animals fed alcohol with PC developed septal fibrosis or cirrhosis, and only 48% ± 9% of their lipocytes were transformed, indicating that PC was indeed the protective compound. Ethanol feeding also resulted in decreased liver phospholipids · and PC, and both were corrected by the supplementation. Furthermore, PC stimulated collagenase activity in cultured lipocytes. This PC consisted of several species, mainly dilinoleoylphosphatidylcholine (40%–52%) and palmitoyl-linoleoylphosphatidylcholine (23%–24%). Only dilinoleoylphosphatidylcholine duplicated the effect of the PC on collagenase. Other species of PC, phosphatidylethanolamine, free fatty acids, or choline were without effect.ConclusionsPC prevents alcohol-induced fibrosis and cirrhosis in nonhuman primates, and dilinoleoylphosphatidylcholine appears to be the active species, possibly by promoting collagen breakdown.