Transcriptional regulation of nitrogen-associated metabolism and growth
Transcriptional regulation of nitrogen-associated metabolism and growth
复制标题
DOI：
10.1038/s41586-018-0656-3
复制
发表时间：
2018-11-08
期刊：
影响因子：
64.8
通讯作者：
Brady, Siobhan M.
中科院分区：
文献类型：
--
作者：
Gaudinier, Allison;Rodriguez-Medina, Joel;Brady, Siobhan M.
氮素是植物生长和基本代谢过程所必需的大量营养素。施用含氮肥料可以提高产量，这是绿色革命中的一个重要因素（1）。然而，过量施用化肥会产生灾难性的影响，如富营养化（2）。更好地了解植物如何调节氮代谢对于提高植物产量和减少肥料过度使用至关重要。在这里，我们提出了一个转录调控网络和21个转录因子，调节结构的根和茎系统响应氮供应的变化。这些转录因子的一个子集的遗传扰动揭示了参与氮代谢的酶的协调转录调控。网络中的转录调节因子通过氮代谢的遗传扰动通过反馈进行转录修饰。这里确定的网络，基因和基因调控模块将证明对提高农业生产力至关重要。
Nitrogen is an essential macronutrient for plant growth and basic metabolic processes. The application of nitrogen-containing fertilizer increases yield, which has been a substantial factor in the green revolution(1). Ecologically, however, excessive application of fertilizer has disastrous effects such as eutrophication(2). A better understanding of how plants regulate nitrogen metabolism is critical to increase plant yield and reduce fertilizer overuse. Here we present a transcriptional regulatory network and twenty-one transcription factors that regulate the architecture of root and shoot systems in response to changes in nitrogen availability. Genetic perturbation of a subset of these transcription factors revealed coordinate transcriptional regulation of enzymes involved in nitrogen metabolism. Transcriptional regulators in the network are transcriptionally modified by feedback via genetic perturbation of nitrogen metabolism. The network, genes and gene-regulatory modules identified here will prove critical to increasing agricultural productivity.