Guidelines for the Care and Welfare of Cephalopods in Research -A consensus based on an initiative by CephRes, FELASA and the Boyd Group
Guidelines for the Care and Welfare of Cephalopods in Research -A consensus based on an initiative by CephRes, FELASA and the Boyd Group
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DOI：
10.1177/0023677215580006
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发表时间：
2015-10-01
影响因子：
2.4
通讯作者：
Andrews, Paul L. R.
中科院分区：
文献类型：
--
作者：
Fiorito, Graziano;Affuso, Andrea;Andrews, Paul L. R.
本文是一项国际倡议的结果，是第一次尝试制定头足类动物（即鹦鹉螺，墨鱼，鱿鱼和章鱼）的护理和福利准则，将这类约700种已知的活无脊椎动物物种纳入指令2010/63/EU。它的目的是为研究人员，动物护理委员会，设施管理人员和动物护理人员提供信息，这将有助于改善头足类动物的护理，以及进行实验程序的方式。涵盖的主题包括：指令对头足类动物研究的影响;项目申请要求和授权程序; 3R原则的应用;危害-效益评估和严重程度分类的必要性。指南和特定物种的要求提供：i。供应、捕获和运输;二.设施的环境特性和设计（例如水质控制、照明要求、振动/噪音敏感度）; iii.住宿和护理（包括水箱设计），动物处理，喂养和环境富集; iv.健康和福利评估（例如，监测生物标志物、身体和行为体征）; v.严重程度评估方法; vi.疾病（原因、预防和治疗）; vii.科学程序、全身麻醉和止痛、人道处死方法和死亡确认。还包括了对操作人员的风险评估以及对护理人员、研究人员和兽医的教育和培训要求。目前使用的主要实验室种属的护理和福利要求的详细方面总结在附录中。知识差距被强调，以促进研究，以加强证据基础，为未来修订这些准则。
This paper is the result of an international initiative and is a first attempt to develop guidelines for the care and welfare of cephalopods (i.e. nautilus, cuttlefish, squid and octopus) following the inclusion of this Class of approximate to 700 known living invertebrate species in Directive 2010/63/EU. It aims to provide information for investigators, animal care committees, facility managers and animal care staff which will assist in improving both the care given to cephalopods, and the manner in which experimental procedures are carried out. Topics covered include: implications of the Directive for cephalopod research; project application requirements and the authorisation process; the application of the 3Rs principles; the need for harm-benefit assessment and severity classification. Guidelines and species-specific requirements are provided on: i. supply, capture and transport; ii. environmental characteristics and design of facilities (e.g. water quality control, lighting requirements, vibration/noise sensitivity); iii. accommodation and care (including tank design), animal handling, feeding and environmental enrichment; iv. assessment of health and welfare (e.g. monitoring biomarkers, physical and behavioural signs); v. approaches to severity assessment; vi. disease (causes, prevention and treatment); vii. scientific procedures, general anaesthesia and analgesia, methods of humane killing and confirmation of death. Sections covering risk assessment for operators and education and training requirements for carers, researchers and veterinarians are also included. Detailed aspects of care and welfare requirements for the main laboratory species currently used are summarised in Appendices. Knowledge gaps are highlighted to prompt research to enhance the evidence base for future revision of these guidelines.