Both daughter cells traffic and exocytose membrane at the cleavage furrow during mammalian cytokinesis
Both daughter cells traffic and exocytose membrane at the cleavage furrow during mammalian cytokinesis
复制标题
DOI：
10.1083/jcb.200712137
复制
发表时间：
2008-06-30
影响因子：
7.8
通讯作者：
Toomre, Derek K.
中科院分区：
文献类型：
--
作者：
Goss, John W.;Toomre, Derek K.
胞质分裂过程中的膜运输还不清楚。我们使用先进的活细胞成像技术来跟踪单个囊泡的胞吐作用，以确定组成性胞吐的膜是否被局部递送到卵裂沟。温度敏感的膜货物蛋白水泡性口炎病毒蛋白-黄色荧光蛋白的超灵敏三维共聚焦延时成像显示，来自两个子细胞的囊泡从高尔基体出来并进入沟，遵循曲线路径。免疫定位和光漂白实验表明，个人囊泡积累在中间体和产生一个储备囊泡池，这是不同的内体和溶酶体隔间。全内反射荧光显微镜成像提供了直接的证据，高尔基体衍生的囊泡从两个子细胞不仅交通到沟区域，但码头和融合，支持对称极化胞吐传递模型。与此相反，定量分析的中体分裂显示的中体残留的遗传一个子细胞，表明胞质分裂是由对称和不对称的阶段。
Membrane trafficking during cytokinesis is not well understood. We used advanced live cell imaging techniques to track exocytosis of single vesicles to determine whether constitutively exocytosed membrane is focally delivered to the cleavage furrow. Ultrasensitive three-dimensional confocal time-lapse imaging of the temperature-sensitive membrane cargo protein vesicular stomatitis virus protein-yellow fluorescent protein revealed that vesicles from both daughter cells traffic out of the Golgi and into the furrow, following curvilinear paths. Immunolocalization and photobleaching experiments indicate that individual vesicles accumulate at the midbody and generate a reserve vesicle pool that is distinct from endosomal and lysosomal compartments. Total internal reflection fluorescence microscopy imaging provided direct evidence that Golgi-derived vesicles from both daughter cells not only traffic to the furrow region but dock and fuse there, supporting a symmetrically polarized exocytic delivery model. In contrast, quantitative analysis of midbody abscission showed inheritance of the midbody remnant by one daughter cell, indicating that cytokinesis is composed of both symmetrical and asymmetrical stages.