Associations of LEP, CRH, ICAM-1, and LINE-1 methylation, measured in saliva, with waist circumference, body mass index, and percent body fat in mid-childhood.
Associations of LEP, CRH, ICAM-1, and LINE-1 methylation, measured in saliva, with waist circumference, body mass index, and percent body fat in mid-childhood.
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DOI：
10.1186/s13148-017-0327-5
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发表时间：
2017
影响因子：
5.7
通讯作者：
Schwartz BS
中科院分区：
文献类型：
--
作者：
Dunstan J;Bressler JP;Moran TH;Pollak JS;Hirsch AG;Bailey-Davis L;Glass TA;Schwartz BS
遗传学解释了人口水平上体重指数的一小部分变异。表观遗传学，通常通过基因甲基化来测量，为理解肥胖风险因素和机制带来了希望。参与者是431名10-15岁的青少年。测量BMI z评分、腰围z评分和体脂百分比。收集唾液样品，并测量四个候选基因或序列（LEP、ICAM-1、CRH和LINE-1）的启动子区域各自在3-4个CpG位点中的甲基化。线性回归用于确定甲基化与肥胖相关结果的关联。校正年龄后，在性别分层分析中，仅肥胖男孩的三种肥胖相关结局与LEP甲基化呈负相关。其他基因或序列的甲基化与肥胖相关的结果没有关联。我们的研究结果与先前的研究一致，这些研究报告了肥胖相关结果与LEP甲基化之间的性别差异，并且也与脂肪组织（循环瘦素的来源）中的预期一致。研究结果表明，唾液可能是青少年表观遗传学研究的可接受组织。本文的在线版本（doi：10.1186/s13148-017-0327-5）包含补充材料，可供授权用户使用。
Genetics explains a small proportion of variance in body mass index at the population level. Epigenetics, commonly measured by gene methylation, holds promise for understanding obesity risk factors and mechanisms. Participants were 431 adolescents aged 10–15 years. BMI z-score, waist circumference z-score, and percent body fat were measured. Saliva samples were collected and methylation of promoter regions of four candidate genes or sequences (LEP, ICAM-1, CRH, and LINE-1) were measured in 3–4 CpG sites each. Linear regression was used to identify associations of methylation with obesity-related outcomes. After adjusting for age, in sex-stratified analysis, the three obesity-related outcomes were negatively associated with LEP methylation in obese boys only. There were no associations of methylation of the other genes or sequences and the obesity-related outcomes. Our results are consistent with prior studies that reported sex differences in associations of obesity-related outcomes with LEP methylation, and also as would be expected in adipose tissue, the source of circulating leptin. The findings suggest that saliva might be an acceptable tissue for epigenetics studies in adolescents. The online version of this article (doi:10.1186/s13148-017-0327-5) contains supplementary material, which is available to authorized users.