Calcium carbonate/CaIP6 nanocomposite particles as gene delivery vehicles for human vascular smooth muscle cells
Calcium carbonate/CaIP6 nanocomposite particles as gene delivery vehicles for human vascular smooth muscle cells
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DOI：
10.1039/c0jm00852d
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发表时间：
2010-01-01
影响因子：
--
通讯作者：
Xu, An-Wu
中科院分区：
文献类型：
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作者：
Cheang, Tuck-yun;Wang, Sheng-ming;Xu, An-Wu
基因治疗为治疗从遗传性疾病到免疫系统疾病和癌症的疾病提供了巨大的机会。许多工作集中在生物活性大分子和纳米颗粒上，它们可以与低分子量药物或DNA分子相互作用形成复合物，并已用于组织再生或基因治疗的治疗因子的局部递送。在这项研究中，无机无定形碳酸钙（ACC）的杂化纳米球功能化与少量的Ca（II）-IP 6化合物（CaIP 6）的大规模制备通过一个简单的气体扩散方法。体外转染实验结果表明，与市售Lipofectamine 2000相比，所制备的功能性碳酸钙/磷酸盐纳米复合粒子具有更高的转染效率和更低的细胞毒性。从这些结果可以开发用于基因递送的替代新载体。此外，功能化无机纳米复合粒子具有生物相容性和生物降解性，因此所制备的杂化纳米球作为一种安全的生物材料具有良好的生物医学应用前景。
Gene therapy provides great opportunities for treating diseases from genetic disorders to immune system diseases and cancer. Much work has focused on bioactive macromolecules and nanoparticles, which can interact with low-molecular-mass drugs or DNA molecules to form complexes, and have been used for local delivery of therapeutic factors for tissue regeneration or gene therapy. In this study, inorganic amorphous calcium carbonate (ACC) hybrid nanospheres functionalized with a small amount of Ca(II)-IP6 compound (CaIP6) were prepared on a large scale by a facile gas-diffusion method. The results of in vitro transfection experiments show that the obtained functional calcium carbonate/phosphate nanocomposite particles had higher transfection efficiency and lower cytotoxicity level than commercial Lipofectamine 2000. From these results alternative new vectors for gene delivery could be developed. In addition, functionalized inorganic nanocomposite particles are biocompatible and biodegradable, thus the as-prepared hybrid nanospheres are promising for biomedical applications as a safe biomaterial.