Glucose transporter isoform 4 is expressed in the syncytiotrophoblast of first trimester human placenta
Glucose transporter isoform 4 is expressed in the syncytiotrophoblast of first trimester human placenta
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DOI：
10.1093/humrep/deh596
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发表时间：
2005-02-01
影响因子：
6.1
通讯作者：
Jansson, T
中科院分区：
文献类型：
--
作者：
Ericsson, A;Hamark, B;Jansson, T
背景：妊娠早期胎盘葡萄糖转运机制知之甚少。本研究的目的是研究葡萄糖转运蛋白（GLUT）亚型1，3和4在早孕绒毛组织中的表达，评估胰岛素对葡萄糖摄取的影响，并与足月妊娠进行比较。研究方法：采用免疫组化、Western blot和逆转录-PCR方法检测妊娠6-13周和足月妊娠滋养层组织中GLUT亚型的表达。胰岛素（300 ng/ml，1小时）对葡萄糖摄取的影响进行了研究绒毛碎片。结果如下：在孕早期，GLUT 1和GLUT 3存在于微绒毛膜和细胞滋养层，GLUT 4存在于合体滋养层（ST）胞质中的核膜中。在滋养层匀浆中鉴定了GLUT 4蛋白（48 kDa）和mRNA。GLUT 1在足月胎盘中表达丰富，而GLUT 3和4在足月胎盘中的表达明显低于孕早期。胰岛素使葡萄糖摄取增加182%（n=6，P
Background: Placental glucose transport mechanisms in early pregnancy are poorly understood. The aims of this study were to investigate the expression of glucose transporter (GLUT) isoforms 1, 3 and 4 in first trimester villous tissue, to assess the effects of insulin on glucose uptake and compare them with term. Methods: The expression of GLUT isoforms was investigated using immunohistochemistry, Western blot and reverse transcription (RT)-PCR in trophoblast tissue from terminations at 6-13 weeks gestation and term. The effects of insulin (300 ng/ml, 1 h) on glucose uptake were studied in villous fragments. Results: In the first trimester, GLUT1 and GLUT3 were present in the microvillous membrane and the cytotrophoblast, and GLUT4 in perinuclear membranes in the cytosol of the syncytiotrophoblast (ST). GLUT4 protein (48 kDa) and mRNA were identified in trophoblast homogenates. Whereas GLUT1 was expressed abundantly in term placenta, the expression of GLUT3 and 4 was markedly lower at term compared with first trimester. Insulin increased glucose uptake by 182% (n=6, P