Basic fibroblast growth factor promotes in vivo muscle regeneration in murine muscular dystrophy
Basic fibroblast growth factor promotes in vivo muscle regeneration in murine muscular dystrophy
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DOI：
10.1016/0304-3940(95)12223-0
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发表时间：
1995-12-29
影响因子：
2.5
通讯作者：
Sebille, A
中科院分区：
文献类型：
--
作者：
Lefaucheur, JP;Sebille, A
由于缺乏肌营养不良蛋白（一种肌膜下蛋白），多药耐药突变小鼠从断奶开始就经历了一轮又一轮的自发性肌纤维坏死再生。肌肉再生很可能是由碱性成纤维细胞生长因子（bFGF），这是可检测到的MDR肌肉再生区域控制。此外，mdr卫星细胞的增殖似乎对培养物中的bFGF特别敏感。我们在第一轮肌肉坏死再生时，在4周龄小鼠的胫骨前肌中注射不同浓度的bFGF，并通过定量组织学评估bFGF对mdx肌肉再生的体内影响。bFGF注射后7天，再生肌纤维的数量显着增加成比例的注射bFGF的浓度。这种效应是由于肌肉卫星细胞的复制增强，如用5-溴-2 '-脱氧尿苷（BrdU）标记增殖细胞所示。这些结果可能提供了一种可能性，通过增加bFGF的可用性，改善肌营养不良蛋白缺乏的肌肉再生。
Due to the lack of dystrophin, a subsarcolemmal protein, mdr mutant mice undergo spontaneous rounds of myofiber necrosis-regeneration from the age of weaning. Muscle regeneration is likely to be controlled by basic fibroblast growth factor (bFGF) which is detectable in regenerating areas of mdr muscles. Moreover, the proliferation of mdr satellite cells seems to be particularly sensitive to bFGF in culture. We injected various concentrations of bFGF in the tibialis anterior muscle of 4-week-old mice at the time of the first round of muscle necrosis-regeneration, and we evaluated the in vivo effects of bFGF on mdx muscle regeneration by quantitative histology. Seven days after bFGF injection, the number of regenerated myofibers was significantly increased proportionally to the injected bFGF concentration. This effect was due to an enhanced replication of muscle satellite cells as shown by labeling the proliferating cells with 5-bromo-2'-deoxyuridine (BrdU). These results may provide a possibility of improving dystrophin-deficient muscle regeneration by increasing the availability of bFGF.