Microscopic analysis of polymerization dynamics with individual actin filaments
Microscopic analysis of polymerization dynamics with individual actin filaments
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DOI：
10.1038/ncb841
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发表时间：
2002-09-01
影响因子：
21.3
通讯作者：
Ishiwata, S
中科院分区：
文献类型：
--
作者：
Fujiwara, I;Takahashi, S;Ishiwata, S
肌动蛋白的聚合-解聚动力学是多种细胞功能的关键过程。许多光谱研究已经在溶液中进行，但对单个肌动蛋白丝的研究才刚刚开始。在这里，我们表明，个别长丝聚合的时间过程由聚合阶段和随后的稳态阶段。在稳态阶段期间，发生在倒刺端部处的伸长和在尖端处的缩短的螺旋铣削过程，其中该过程的两个组分以大致相同的速率进行。在稳态阶段的长丝的长度波动的时间相关性表明，聚合-解聚动力学遵循扩散（随机）的过程，这不能解释为简单的缔合和解离的单体在两端的长丝。
The polymerization-depolymerization dynamics of actin is a key process in a variety of cellular functions. Many spectroscopic studies have been performed in solution, but studies on single actin filaments have just begun. Here, we show that the time course of polymerization of individual filaments consists of a polymerization phase and a subsequent steady-state phase. During the steady-state phase, a treadmilling process of elongation at the barbed end and shortening at the pointed end occurs, in which both components of the process proceed at approximately the same rate. The time correlation of length fluctuation of the filaments in the steady-state phase showed that the polymerization-depolymerization dynamics follow a diffusion (stochastic) process, which cannot be explained by simple association and dissociation of monomers at both ends of the filaments.