Cartilage, SOX9 and notch signals in chondrogenesis
Cartilage, SOX9 and notch signals in chondrogenesis
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DOI：
10.1111/j.1469-7580.2006.00630.x
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发表时间：
2006-10-01
影响因子：
2.4
通讯作者：
Tew, Simon R.
中科院分区：
文献类型：
--
作者：
Hardingham, Timothy E.;Oldershaw, Rachel A.;Tew, Simon R.
腕关节修复是一项持续的医学挑战。组织工程的解决方案，这个问题依赖于适当分化的细胞在足够数量的可用性。本文综述了原代人关节软骨细胞和间充质干细胞的潜力，以履行这一作用。软骨细胞已经用含有转录因子SOX 9的逆转录病毒转导，其允许细胞对三维培养系统、生长因子刺激和缺氧培养条件的反应大大改善。人类间充质干细胞已分化成软骨细胞使用完善的方法，和Notch信号通路已被详细研究，以确定其在这一过程中的作用。这两种方法都提供了对这些体外系统的见解，这些系统对于理解和设计未来的软骨再生策略是非常宝贵的。
Cartilage repair is an ongoing medical challenge. Tissue engineered solutions to this problem rely on the availability of appropriately differentiated cells in sufficient numbers. This review discusses the potential of primary human articular chondrocytes and mesenchymal stem cells to fulfil this role. Chondrocytes have been transduced with a retrovirus containing the transcription factor SOX9, which permits a greatly improved response of the cells to three-dimensional culture systems, growth factor stimulation and hypoxic culture conditions. Human mesenchymal stem cells have been differentiated into chondrocytes using well-established methods, and the Notch signalling pathway has been studied in detail to establish its role during this process. Both approaches offer insights into these in vitro systems that are invaluable to understanding and designing future cartilage regeneration strategies.