TETRAD ANALYSIS POSSIBLE IN ARABIDOPSIS WITH MUTATION OF THE QUARTET (QRT) GENES
TETRAD ANALYSIS POSSIBLE IN ARABIDOPSIS WITH MUTATION OF THE QUARTET (QRT) GENES
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DOI：
10.1126/science.8197459
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发表时间：
1994-06-03
期刊：
影响因子：
56.9
通讯作者：
DAVIS, RW
中科院分区：
文献类型：
--
作者：
PREUSS, D;RHEE, SY;DAVIS, RW
两个拟南芥基因，QRT1和QRT2，需要在正常发育过程中的花粉分离。在qrt突变体中，花粉母细胞的四个减数分裂产物的外壁融合，花粉粒以四分体的形式释放。花粉可育，细胞质花粉内含物分散。用单个四分体授粉通常会产生四粒种子，遗传分析证实标记基因座在这些四分体内以2：2的比例分离。这些突变允许在拟南芥中进行四分体分析，并定义花粉细胞壁发育的步骤。
Two Arabidopsis thaliana genes, QRT1 and QRT2, are required for pollen separation during normal development. In qrt mutants, the outer walls of the four meiotic products of the pollen mother cell are fused, and pollen grains are released in tetrads. Pollen is viable and fertile, and the cytoplasmic pollen contents are discrete. Pollination with a single tetrad usually yields four seeds, and genetic analysis confirmed that marker loci segregate in a 2:2 ratio within these tetrads. These mutations allow tetrad analysis to be performed in Arabidopsis and define steps in pollen cell wall development.