A Major Fimbrilin Variant of Mfa1 Fimbriae in Porphyromonas gingivalis
A Major Fimbrilin Variant of Mfa1 Fimbriae in Porphyromonas gingivalis
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DOI：
10.1177/0022034515588275
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发表时间：
2015-08-01
影响因子：
7.6
通讯作者：
Yoshimura, F.
中科院分区：
文献类型：
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作者：
Nagano, K.;Hasegawa, Y.;Yoshimura, F.
已知牙周病原体牙龈卟啉单胞菌表达2种不同类型的菌毛：FimA和Mfa1菌毛。然而，我们以前报道过，在牙龈卟啉单胞菌菌株中发现了菌毛样结构，其中既没有检测到FimA也没有检测到Mfa1菌毛。在这项研究中，我们确定了菌株的细菌裂解物中的主要蛋白质，已被报道为牙龈卟啉单胞菌的53 kDa主要外膜蛋白（53 K蛋白），随后被报道为新型菌毛的主要菌毛蛋白。对该菌株的染色体DNA测序表明，53k基因（编码53K蛋白）位于与ATCC 33277型菌株的mfa1基因（编码Mfa1蛋白，其是Mfa1菌毛的主要菌毛蛋白）相对应的基因座上。然而，53K和Mfa1蛋白显示出低氨基酸序列同源性和不同的抗原性。在84株牙龈卟啉单胞菌中有34株（41%）检测到53K蛋白，而在44%的菌株中检测到Mfa1蛋白。没有菌株同时表达53K和Mfa1蛋白。此外，菌毛在53k和mfa1基因互换的突变体中正常表达。这些结果表明，53K蛋白是牙龈卟啉单胞菌中Mfa1菌毛的另一个主要菌毛蛋白。
The periodontal pathogen Porphyromonas gingivalis is known to express 2 distinct types of fimbriae: FimA and Mfa1 fimbriae. However, we previously reported that fimbria-like structures were found in a P. gingivalis strain in which neither FimA nor Mfa1 fimbriae were detected. In this study, we identified a major protein in the bacterial lysates of the strain, which has been reported as the 53-kDa major outer membrane protein of P. gingivalis (53K protein) and subsequently reported as a major fimbrilin of a novel-type fimbria. Sequencing of the chromosomal DNA of the strain showed that the 53k gene (encoding the 53K protein) was located at a locus corresponding to the mfa1 gene (encoding the Mfa1 protein, which is a major fimbrilin of Mfa1 fimbriae) of the ATCC 33277 type strain. However, the 53K and Mfa1 proteins showed a low amino acid sequence homology and different antigenicity. The 53K protein was detected in 34 of 84 (41%) P. gingivalis strains, while the Mfa1 protein was detected in 44% of the strains. No strain expressed both 53K and Mfa1 proteins. Additionally, fimbriae were normally expressed in mutants in which the 53k and mfa1 genes were interchanged. These results indicate that the 53K protein is another major fimbrilin of Mfa1 fimbriae in P. gingivalis.