Large-scale Proteomics Combined with Transgenic Experiments Demonstrates An Important Role of Jasmonic Acid in Potassium Deficiency Response in Wheat and Rice.
Large-scale Proteomics Combined with Transgenic Experiments Demonstrates An Important Role of Jasmonic Acid in Potassium Deficiency Response in Wheat and Rice.
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DOI：
10.1074/mcp.ra117.000032
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发表时间：
2017-11
期刊：
影响因子：
--
通讯作者：
Kang G
中科院分区：
文献类型：
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作者：
Li G;Wu Y;Liu G;Xiao X;Wang P;Gao T;Xu M;Han Q;Wang Y;Guo T;Kang G
钾（K+）是植物体内含量最丰富的无机阳离子，为了减少钾肥投入，培育优质高效作物，K+缺乏的分子机理研究受到了广泛关注。然而，植物对K+缺乏反应的分子机制仍然知之甚少。在这项研究中，2周龄的面包小麦幼苗水培生长在Hoagland解决方案被转移到K+-无条件下8天，他们的根和叶蛋白质组谱进行了评估，使用iTRAQ蛋白质组方法。超过4000个独特的蛋白质被确定，和818 K+响应蛋白种类显示出显着差异的丰度。差异表达的蛋白质种类与不同的功能，并表现出器官特异性差异。与激素合成相关的差异表达蛋白主要参与茉莉酸（JA）的合成，JA合成相关酶的丰度上调可导致JA浓度升高。在缺钾条件下，JA合成的关键酶丙二烯氧化物合酶（AOS）的表达显著增加，其过表达可显著提高K+和JA浓度，改变某些K+响应蛋白基因的转录水平，增强水稻对低K+或缺K+的耐受性。此外，水稻AOS突变体（osaos）对低K+或缺K+表现出更高的敏感性。我们的研究结果可以突出JA在K+缺乏中的重要性，并暗示植物对K+缺乏的反应的分子过程网络。
Potassium (K+) is the most abundant inorganic cation in plants, and molecular dissection of K+ deficiency has received considerable interest in order to minimize K+ fertilizer input and develop high quality K+-efficient crops. However, the molecular mechanism of plant responses to K+ deficiency is still poorly understood. In this study, 2-week-old bread wheat seedlings grown hydroponically in Hoagland solution were transferred to K+-free conditions for 8 d, and their root and leaf proteome profiles were assessed using the iTRAQ proteome method. Over 4000 unique proteins were identified, and 818 K+-responsive protein species showed significant differences in abundance. The differentially expressed protein species were associated with diverse functions and exhibited organ-specific differences. Most of the differentially expressed protein species related to hormone synthesis were involved in jasmonic acid (JA) synthesis and the upregulated abundance of JA synthesis-related enzymes could result in the increased JA concentrations. Abundance of allene oxide synthase (AOS), one key JA synthesis-related enzyme, was significantly increased in K+-deficient wheat seedlings, and its overexpression markedly increased concentrations of K+ and JA, altered the transcription levels of some genes encoding K+-responsive protein species, as well as enhanced the tolerance of rice plants to low K+ or K+ deficiency. Moreover, rice AOS mutant (osaos) exhibited more sensitivity to low K+ or K+ deficiency. Our findings could highlight the importance of JA in K+ deficiency, and imply a network of molecular processes underlying plant responses to K+ deficiency.