Modulation of primary cell function of host Pseudomonas bacteria by the conjugative plasmid pCAR1
Modulation of primary cell function of host Pseudomonas bacteria by the conjugative plasmid pCAR1
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DOI：
10.1111/1462-2920.12515
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发表时间：
2015-01-01
影响因子：
5.1
通讯作者：
Nojiri, Hideaki
中科院分区：
文献类型：
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作者：
Takahashi, Yurika;Shintani, Masaki;Nojiri, Hideaki
利用接合质粒pCAR 1在恶臭假单胞菌KT 2440、铜绿假单胞菌PAO 1和荧光假单胞菌Pf 0 -1三种不同的假单胞菌宿主中进行了质粒携带对宿主细胞的影响的综合分析。质粒运输减少宿主健身，游泳运动，并抵抗渗透压或pH值的压力。质粒携带引起宿主TCA循环中初级代谢能力的改变。通过生长期依赖性转录组分析，确定了与质粒携带相关的三种宿主中的差异转录基因。质粒携带通常在过渡期和早期稳定期对宿主转录组表现出更大的影响。KT 2440和PAO 1之间的转录组改变相似。两株菌中编码核糖体蛋白、F-型ATP酶和RNAP核心的基因的数量在稳定期早期的质粒携带中没有受到足够的抑制。这些反应可能导致宿主适应性、运动性和应激抗性的降低。宿主对质粒携带的特异性反应是假定的原噬菌体或外源DNA区域上的基因的转录变化。对宿主表型和转录组的影响程度在KT 2440中同样最大，在Pf 0 -1中最低。这些发现表明，宿主细胞的功能受到质粒携带的积极调控。
The impacts of plasmid carriage on the host cell were comprehensively analysed using the conjugative plasmid pCAR1 in three different Pseudomonas hosts, P.putidaKT2440, P.aeruginosaPAO1 and P.fluorescensPf0-1. Plasmid carriage reduced host fitness, swimming motility, and resistance to osmotic or pH stress. Plasmid carriage brought about alterations in primary metabolic capacities in the TCA cycle of the hosts. Differentially transcribed genes in the three hosts associated with plasmid carriage were identified by growth phase-dependent transcriptome analyses. Plasmid carriage commonly showed a greater effect on the host transcriptome at the transition and early stationary phases. The transcriptome alterations were similar between KT2440 and PAO1. Transcriptions of numbers of genes encoding ribosomal proteins, F-type ATPase, and RNAP core in both strains were not suppressed enough in the early stationary phase by plasmid carriage. These responses may have been responsible for the reduction in host fitness, motility and stress resistances. Host-specific responses to plasmid carriage were transcriptional changes of genes on putative prophage or foreign DNA regions. The extents of the impacts on host phenotypes and transcriptomes were similarly greatest in KT2440 and lowest in Pf0-1. These findings suggest that host cell function was actively regulated by plasmid carriage.