Study on the relationship between plasma nitrite and nitrate level and salt sensitivity in human hypertension - Modulation of nitric oxide synthesis by salt intake
Study on the relationship between plasma nitrite and nitrate level and salt sensitivity in human hypertension - Modulation of nitric oxide synthesis by salt intake
复制标题
DOI：
10.1161/01.cir.101.8.856
复制
发表时间：
2000-02-29
期刊：
影响因子：
37.8
通讯作者：
Okumura, K
中科院分区：
文献类型：
--
作者：
Fujiwara, N;Osanai, T;Okumura, K
背景-在动物模型中，高盐摄入抑制了外周阻力血管中一氧化氮（NO）的作用。我们测试的假设，内源性NO的调制与盐的敏感性在人类hypertension.Methods和Results-Inpatients原发性高血压（n=24）维持在正常盐饮食（12 g/d NaCl）3天。低盐饮食（2 g）、高盐饮食（20 - 23 g）和低盐饮食7天。血压正常的受试者（n=16）维持前2盐饮食。将盐负荷后24 h平均血压升高>5%的高血压患者分为1组（n=8），其余患者分为2组（n=8）。（n=16）。硝酸盐加亚硝酸盐（NO）通过Griess法测定。和不对称二甲基精氨酸（ADMA）。正常盐饮食期间，I组血浆NO_3水平低于2组和血压正常组。盐加载后？血浆NO水平下降，后又逆转。第二个限制盐。血浆ADMA水平在盐负荷后升高，限盐后降低。盐负荷和限制后血浆NO水平的变化与血压（r=-0.59，P =0.0007）和血浆ADMA水平（r=-0.64，P =0.003）呈负相关。人类高血压盐敏感性的机制，可能是通过ADMA的变化。
Background-High salt intake suppresses the effect of nitric oxide (NO) in the peripheral resistance vessels in animal models. We tested the hypothesis that the modulation of endogenous NO is related to salt sensitivity in human hypertension.Methods and Results-Inpatients with essential hypertension (n=24) were maintained on a normal-salt diet (12 g/d NaCl) for 3 days. a low-salt diet (2 g), a high-salt diet (20 to 23 g), and a low-salt diet for 7 days. Normotensive subjects (n=16) were maintained on the first 2 salt diets. The hypertensive patients whose average 24-hour blood pressure was increased by >5% by salt loading were assigned to group 1 (n=8) and the others to group ? (n=16). Nitrate plus nitrite (NO,) was measured by the Griess method. and asymmetrical dimethylarginine (ADMA) by high-performance liquid chromatography. The plasma NO, level during the normal-salt diet was lower in group I than in group 2 and the normotensive group. After salt loading? the plasma NO, level was decreased and reversed after. the second salt restriction. Plasma ADMA level was increased after salt loading and decreased after salt restriction. The change in plasma NOx level was correlated inversely with those in blood pressure (r=-0.59, P=0.0007) and plasma ADMA level (r=-0.64, P=0.003) after salt loading and restriction.Conclusions-Modulation of NO synthesis by salt intake may be involved in a. mechanism for salt sensitivity in human hypertension, presumably via the change in ADMA.