Trehalose Changes Hydraulic Conductance of Tissue-cultured Soybean Embryos
Trehalose Changes Hydraulic Conductance of Tissue-cultured Soybean Embryos
复制标题
海藻糖改变组织培养大豆胚胎的水力传导
DOI：
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发表时间：
2000
期刊：
影响因子：
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通讯作者：
H. Nonami
中科院分区：
文献类型：
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作者：
T. Ikeda;M. Iwaya‐Inoue;T. Fukuyama;H. Nonami
对组织培养大豆（Glycine max [L.]梅尔）研究了在含海藻糖的培养基上生长的胚胎。当蔗糖和/或海藻糖浓度改变时，培养基的水势变化范围为-0.13 ~-0.65MPa。细胞壁伸展性远大于导水率，而有效膨压远小于生长诱导水势，表明导水率和生长诱导水势是组织培养条件下大豆胚生长的主要调控因子。在含海藻糖的培养基上生长的大豆胚的导水率比在含蔗糖的培养基上生长的导水率小。当蔗糖和海藻糖都加入到培养基中，水力传导成为一个中间体之间的只有蔗糖和只有海藻糖的治疗。结果表明，海藻糖可降低大豆胚的导水率，导致大豆胚的生长迟缓。
The hydraulic properties of tissue-cultured soybean (Glycine max [L.] Merr.) embryos grown on trehalose-containing culture media were investigated. Water potentials of culture media ranged from -0.13 to -0.65MPa when sucrose and/or trehalose concentrations were altered. The wall extensibility was greatly larger than hydraulic conductance, and the size of the effective turgor was much smaller than that of the growth-induced water potential, indicating that hydraulic conductance and the growth-induced water potential were predominantly regulating growth of soybean embryos under tissue culture conditions. The hydraulic conductance of soybean embryos grown on trehalose-containing media became smaller than that in sucrose-containing media. When both sucrose and trehalose were added to the culture media, the hydraulic conductance became an intermediate between only sucrose-containing and only trehalose-containing treatments. It is suggested that trehalose might reduce hydraulic conductance in soybean embryos, resulting in growth retardation of soybean embryos.