Inheritance and organisation of the mitochondrial genome differ between two Saccharomyces yeasts
Inheritance and organisation of the mitochondrial genome differ between two Saccharomyces yeasts
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DOI：
10.1016/s0022-2836(02)00037-2
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发表时间：
2002-05-03
影响因子：
5.6
通讯作者：
Piskur, J
中科院分区：
文献类型：
--
作者：
Petersen, RF;Langkjær, RB;Piskur, J
小阳性酵母菌大致可分为狭义酵母菌群（包括酿酒酵母菌）和狭义酵母菌群（包括castelli酵母菌）；后者最近研究了其传播和线粒体基因组的组织。携带点突变的酿酒葡萄球菌线粒体分子（mtDNA）在遗传杂交中表现为酿酒葡萄球菌相应的点突变体。尽管castellii以与酿酒葡萄相似的方式产生了自发的小突变体，但小突变体表现出不同的遗传模式。在与野生型菌株的杂交中，大部分castellii petites是中性的，抑制型petites的抑制率从未超过50%。这两种酵母的mtDNA分子结构也不同。测定了castellii S. mtDNA的25753 bp序列，两种酵母的编码潜力相似。然而，castellii的基因间序列要短得多，并且不包含与酿酒葡萄球菌具有生物活性的基因间序列同源的序列，如ori/rep/tra，这些序列负责酿酒葡萄球菌的超抑制小表型。一个抑制性castellii突变体CA38的结构也被确定。显然，一个短的直接基因间重复参与了这个微小的mtDNA分子的产生。(C) 2002 Elsevier Science Ltd.版权所有。
Petite-positive Saccharomyces yeasts can be roughly divided into the sensu stricto, including Saccharomyces cerevisiae, and sensu lato group, including Saccharomyces castellii; the latter was recently studied for transmission and the organisation of its mitochondrial genome. S. castellii mitochondrial molecules (mtDNA) carrying point mutations, which confer antibiotic resistance, behaved in genetic crosses as the corresponding point mutants of S. cerevisiae. While S. castellii generated spontaneous petite mutants in a similar way as S. cerevisiae, the petites exhibited a different inheritance pattern. In crosses with the wild type strains a majority of S. castellii petites was neutral, and the suppressivity in suppressive petites was never over 50%. The two yeasts also differ in organisation of their mtDNA molecules. The 25,753 bp sequence of S. castellii mtDNA was determined and the coding potential of both yeasts is similar. However, the S. castellii intergenic sequences are much shorter and do not contain sequences homologous to the S. cerevisiae biologically active intergenic sequences, as ori/rep/tra, which are responsible for the hyper-suppressive petite phenotype found in S, cerevisiae. The structure of one suppressive S. castellii mutant, CA38, was also determined. Apparently, a short direct intergenic repeat was involved in the generation of this petite mtDNA molecule. (C) 2002 Elsevier Science Ltd. All rights reserved.