Comprehensive Analysis of Reproductive ADAMs: Relationship of ADAM4 and ADAM6 with an ADAM Complex Required for Fertilization in Mice
Comprehensive Analysis of Reproductive ADAMs: Relationship of ADAM4 and ADAM6 with an ADAM Complex Required for Fertilization in Mice
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DOI：
10.1095/biolreprod.108.073700
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发表时间：
2009-05-01
影响因子：
3.6
通讯作者：
Cho, Chunghee
中科院分区：
文献类型：
--
作者：
Han, Cecil;Choi, Eunyoung;Cho, Chunghee
在男性生殖组织中表达的解整合素和金属蛋白酶 (ADAM) 家族成员在系统发育上分为三个主要组。在本研究中，我们分析了一组中的 6 个 ADAM（ADAM 4、6、24、26、29 和 30），其功能很大程度上未知。我们的结果表明，大多数 ADAM 在精子成熟过程中经历了独特的加工，并且位于精子头部的表面。我们发现，在各种受精过程中有缺陷的 Adam2 和 Adam3 敲除精子中，ADAM4 和 ADAM6 的水平显着降低。我们观察到 Adam3 缺失小鼠中 ADAM4 的过早加工。此外，我们还获得了睾丸中 ADAM6 与 ADAM2 和 ADAM3 形成复合物的结果。总而言之，这些结果揭示了 ADAM4 和 ADAM6 参与了在受精过程中发挥作用的生殖 ADAM 系统。
A Disintegrin And Metalloprotease (ADAM) family members expressed in male reproductive tissues are divided phylogenetically into three major groups. In the present study, we analyzed six ADAMs in one of the groups (ADAMs 4, 6, 24, 26, 29, and 30) of which function is largely unknown. Our results showed that most of the ADAMs undergo unique processing during sperm maturation and are located at the surface of sperm head. We found that the levels of ADAM4 and ADAM6 are dramatically reduced in Adam2 and Adam3 knockout sperm defective in various fertilization processes. We observed premature processing of ADAM4 in the Adam3-null mice. Furthermore, we obtained a result showing complex formation of ADAM6 with ADAM2 and ADAM3 in testis. Taken together, these results disclose involvement of ADAM4 and ADAM6 in a reproductive ADAM system that functions in fertilization.