Studies on Murine IgE with Monoclonal Antibodies
Studies on Murine IgE with Monoclonal Antibodies
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鼠IgE单克隆抗体研究
DOI：
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发表时间：
1988
期刊：
影响因子：
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通讯作者：
K. Okumura
中科院分区：
文献类型：
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作者：
T. Hirano;H. Miyajima;H. Kitagawa;N. Watanabe;M. Azuma;O. Taniguchi;H. Hashimoto;S. Hirose;H. Yagita;S. Furusawa;Z. Óváry;K. Okumura
通过将免疫的大鼠脾细胞与非分泌性小鼠骨髓瘤细胞融合来构建大鼠单克隆抗体。选择两种单克隆抗体（6HD5和HMK-12）进行进一步研究。两者均与不同特异性和不同同种型的各种 IgE 分子发生反应，但不与其他同种型的免疫球蛋白和轻链发生反应。因此，这些抗体是抗同种型 (IgE)，而不是抗同种异型或抗独特型。竞争研究表明，这些抗体识别 FcR epsilon 片段上的不同表位。使用这些单克隆抗体开发了一种用于定量鼠 IgE 的灵敏 ELISA；检测血清 IgE 水平的灵敏度在 2 至 250 ng/ml 之间。酶联免疫吸附测定 (ELISA) 和被动皮肤过敏反应 (PCA) 活性测定的蛋白质含量与蛋白质含量具有良好的相关性。两种单克隆抗体均用于研究 IgE 抗体引起的过敏反应。两者都可以抑制 PCA 反应，并且都可以引发反向 PCA 反应。
Rat monoclonal antibodies were constructed by fusion of immunized rat spleen cells with a nonsecreting mouse myeloma cell. Two monoclonal antibodies (6HD5 and HMK-12) were selected for further study. Both reacted with various IgE molecules of different specificities and different allotypes, but did not react with immunoglobulins of other isotypes and with light chains. These antibodies were therefore anti-isotypic (IgE) and not anti-allotypic or anti-idiotypic. It was shown by competition studies that these antibodies recognize different epitopes on the FcR epsilon fragment. A sensitive ELISA for the quantitation of murine IgE was developed with these monoclonal antibodies; the sensitivity was between 2 and 250 ng/ml for detection of serum IgE levels. Good correlation was obtained with protein amounts as determined by enzyme-linked immunosorbent assay (ELISA) and passive cutaneous anaphylaxis (PCA) activities. Both monoclonal antibodies were used to study anaphylactic reactions elicited by IgE antibodies. Both could inhibit PCA reactions and both could elicit reverse PCA reactions.