Expression of proteinase-activated receptor-2 in human pancreatic cancer: a possible relation to cancer invasion and induction of fibrosis.
Expression of proteinase-activated receptor-2 in human pancreatic cancer: a possible relation to cancer invasion and induction of fibrosis.
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人胰腺癌中蛋白酶激活受体 2 的表达：与癌症侵袭和诱导纤维化的可能关系。
DOI：
10.3892/ijo.22.2.295
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发表时间：
2003
影响因子：
5.2
通讯作者：
M. Ogawa
中科院分区：
文献类型：
--
作者：
O. Ikeda;H. Egami;T. Ishiko;S. Ishikawa;H. Kamohara;H. Hidaka;S. Mita;M. Ogawa
蛋白酶激活受体-2（PAR-2）是一种G蛋白偶联受体，可被胰蛋白酶裂解和激活。为了阐明PAR-2在人胰腺癌中的存在，使用5种人胰腺癌细胞系，通过RT-PCR、免疫印迹和免疫细胞化学分析PAR-2的表达。采用免疫组织化学方法检测PAR-2在胰腺癌组织和非癌组织中的表达，探讨其生物学意义。PAR-2的存在被证实在所有5个胰腺癌细胞系和所有21个石蜡包埋的标本从人胰腺癌检查。PAR-2在浸润型组织中的表达高于扩张型组织。此外，PAR-2在伴有严重纤维化的组织中的表达显著升高。即使在同一标本中，纤维化程度较重的部位免疫反应强度也较轻的部位强。同样，PAR-2在慢性胰腺炎伴重度纤维化患者中的表达高于轻度纤维化患者。综上所述，这些结果表明PAR-2的激活参与了人类胰腺癌的侵袭和纤维化的诱导。
The proteinase-activated receptor-2 (PAR-2) is a G protein-coupled receptor that is cleaved and activated by trypsin. To clarify the presence of PAR-2 in human pancreatic cancer, the expression of PAR-2 was analyzed by RT-PCR, immunoblotting and immunocytochemistry using 5 human pancreatic cancer cell lines. And to evaluate the biological significance, immunohistochemical expression of PAR-2 in malignant and non-malignant human pancreatic tissues was examined using paraffin-embedded sections. The presence of PAR-2 was confirmed in all 5 pancreatic cancer cell lines and all 21 paraffin-embedded specimens from human pancreatic cancer examined. The expression of PAR-2 was found to be higher in the tissues with infiltrative growth pattern than those with expansive growth pattern. Moreover, significantly higher expression of PAR-2 was observed in the tissues which were accompanied by severe fibrosis. Even in the same specimen, the intensity of immunoreactivity tended to be stronger in the part with severe fibrosis than that with mild fibrosis. Similarly, the higher expression of PAR-2 was observed in chronic pancreatitis with severe fibrosis than with mild fibrosis. Taken together, these results suggest that the activation of PAR-2 is involved in cancer invasion and the induction of fibrosis in human pancreatic cancer.