Direct electrochemistry of glucose oxidase and biosensing for glucose based on boron-doped carbon nanotubes modified electrode
Direct electrochemistry of glucose oxidase and biosensing for glucose based on boron-doped carbon nanotubes modified electrode
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基于硼掺杂碳纳米管修饰电极的葡萄糖氧化酶直接电化学及葡萄糖生物传感
DOI：
10.1016/j.bios.2007.11.009
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发表时间：
2008-03-14
影响因子：
12.6
通讯作者：
Yao, Shouzhuo
中科院分区：
文献类型：
--
作者：
Deng, Chunyan;Chen, Jinhua;Yao, Shouzhuo
由于其独特的物理化学性质，掺杂碳纳米管现在是非常有吸引力的和重要的纳米材料在生物分析应用。本文以葡萄糖氧化酶（GOD）为模型酶，采用循环伏安法研究了硼掺杂碳纳米管/玻碳（BCNTs/GC）电极上GOD的直接电化学行为。在0.1M磷酸盐缓冲溶液（pH6.98）中，通过蛋白质与电极之间的直接电子转移，在BCNTs基酶电极上观察到一对固定化GOD的准可逆氧化还原峰。作为葡萄糖分析的新平台，基于BCNTs/GC电极的新型葡萄糖生物传感器的灵敏度为111.57 μ A mM（-1）cm（-2），线性范围为0.05 ~ 0.3 mM，检测限为0.01 mM（S/N = 3）。此外，BCNTs修饰电极具有良好的稳定性，对常见共存的尿酸和抗坏血酸具有良好的抗干扰能力。这表明硼掺杂碳纳米管是一种很好的用于氧化还原活性酶直接电化学及构建相关酶生物传感器的候选材料。(c)2007 Elsevier B. V.保留所有权利。
Due to their unique physicochemical properties, doped carbon nanotubes are now extremely attractive and important nanomaterials in bioanalytical applications. In this work, selecting glucose oxidase (GOD) as a model enzyme, we investigated the direct electrochemistry of GOD based on the B-doped carbon nanotubes/glassy carbon (BCNTs/GC) electrode with cyclic voltammetry. A pair of well-defined, quasi-reversible redox peaks of the immobilized GOD was observed at the BCNTs based enzyme electrode in 0.1 M phosphate buffer solution (pH 6.98) by direct electron transfer between the protein and the electrode. As a new platform in glucose analysis, the new glucose biosensor based on the BCNTs/GC electrode has a sensitivity of 111.57 mu A mM(-1) cm(-2), a linear range from 0.05 to 0.3 mM and a detection limit of 0.01 mM (S/N = 3). Furthermore, the BCNTs modified electrode exhibits good stability and excellent anti-interferent ability to the commonly co-existed uric acid and ascorbic acid. These indicate that boron-doped carbon nanotubes are the good candidate material for the direct electrochemistry of the redox-active enzyme and the construction of the related enzyme biosensors. (c) 2007 Elsevier B.V. All rights reserved.