Aerobic training stimulates growth and promotes disease resistance in Atlantic salmon (Salmo salar)
Aerobic training stimulates growth and promotes disease resistance in Atlantic salmon (Salmo salar)
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DOI：
10.1016/j.cbpa.2011.06.013
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发表时间：
2011-10-01
影响因子：
2.3
通讯作者：
Takle, Harald
中科院分区：
文献类型：
--
作者：
Castro, Vicente;Grisdale-Helland, Barbara;Takle, Harald
提高鱼类健壮性对减少养殖中的鱼类损失至关重要。虽然以前没有检查，我们假设有氧训练，如人类研究所示，可以加强大西洋鲑鱼（Salina太阳能）的抗病能力。因此，我们在两种不同的有氧训练方案下对鲑鱼幼鱼进行了6周的训练;连续强度训练（CT; 0.8 bl s（-1））和间歇训练（IT; 0.8 bl s（1）16 h和1.0 bl s（-1）8 h），并将其与未经训练的对照组（C; 0.05 bl s（-1））进行了比较。采用IPN病毒攻击试验评价耐力训练对抗病能力的影响，同时采用qPCR和微阵列基因表达分析表征心脏免疫调节作用。此外，游泳性能和生长参数进行了研究。在IPN攻击后，IT鱼（74%）的存活率高于CT鱼（64%）或C鱼（61%）。虽然CT和IT组在IPN攻击测试之前显示出较低的TNF-α，IL-1 β和IL-6的心脏转录水平，但IT鱼显示出参与免疫反应和已知影响抗病性的其他过程的基因的最强调节。CT和IT制度导致更好的增长相比，控制鱼，CT鱼开发一个更好的游泳效率在训练过程中。总的来说，间歇有氧训练改善了大西洋鲑鱼的生长并增加了其健壮性，表现为更好的抗病性，我们发现这与心脏转录组上相关基因类的调节有关。(C)2011 Elsevier Inc. All rights reserved.
Improving fish robustness is of utmost relevance to reducing fish losses in farming. Although not previously examined, we hypothesized that aerobic training, as shown for human studies, could strengthen disease resistance in Atlantic salmon (Salina solar). Thus, we exercised salmon pre-smolts for 6 weeks at two different aerobic training regimes; a continuous intensity training (CT; 0.8 bl s(-1))and an interval training (IT; 0.8 bl s (1) 16 h and 1.0 bl s(-1) 8 h) and compared them with untrained controls (C; 0.05 bl s(-1)). The effects of endurance training on disease resistance were evaluated using an IPN virus challenge test, while the cardiac immune modulatory effects were characterized by qPCR and microarray gene expression analyses. In addition, swimming performance and growth parameters were investigated. Survival after the IPN challenge was higher for IT (74%) fish than for either CT (64%) or C (61%) fish. While both CT and IT groups showed lower cardiac transcription levels of TNF-alpha, IL-1 beta and IL-6 prior to the IPN challenge test, IT fish showed the strongest regulation of genes involved in immune responses and other processes known to affect disease resistance. Both CT and IT regimes resulted in better growth compared with control fish, with CT fish developing a better swimming efficiency during training. Overall, interval aerobic training improved growth and increased robustness of Atlantic salmon, manifested by better disease resistance, which we found was associated with a modulation of relevant gene classes on the cardiac transcriptome. (C) 2011 Elsevier Inc. All rights reserved.