Rab8b Regulates Transport of West Nile Virus Particles from Recycling Endosomes
Rab8b Regulates Transport of West Nile Virus Particles from Recycling Endosomes
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DOI：
10.1074/jbc.m115.712760
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发表时间：
2016-03-18
影响因子：
4.8
通讯作者：
Sawa, Hirofumi
中科院分区：
文献类型：
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作者：
Kobayashi, Shintaro;Suzuki, Tadaki;Sawa, Hirofumi
西尼罗病毒（WNV）颗粒在内质网（ER）中组装并出芽，并通过分泌途径从感染细胞中分泌。然而，与这些步骤相关的宿主因素尚未完全理解。Rab蛋白属于Ras超家族，在调节囊泡运输的许多方面发挥重要作用。在这项研究中，我们试图确定哪些Rab蛋白参与新生WNV颗粒的细胞内运输。RNAi分析显示Rab8b在WNV颗粒释放中起作用。我们发现Rab8和WNV抗原在WNV感染的人神经母细胞瘤细胞中共定位，并且WNV感染增强Rab8在细胞中的表达。此外，Rab8b缺陷细胞上清液中WNV颗粒的量与野生型细胞相比显著降低。我们还证明了WNV颗粒在WNV感染细胞的再循环内体中积累。总之，这些结果表明Rab8b参与WNV颗粒从再循环内体到质膜的运输。
West Nile virus (WNV) particles assemble at and bud into the endoplasmic reticulum (ER) and are secreted from infected cells through the secretory pathway. However, the host factor related to these steps is not fully understood. Rab proteins, belonging to the Ras superfamily, play essential roles in regulating many aspects of vesicular trafficking. In this study, we sought to determine which Rab proteins are involved in intracellular trafficking of nascent WNV particles. RNAi analysis revealed that Rab8b plays a role in WNV particle release. We found that Rab8 and WNV antigen were colocalized in WNV-infected human neuroblastoma cells, and that WNV infection enhanced Rab8 expression in the cells. In addition, the amount of WNV particles in the supernatant of Rab8b-deficient cells was significantly decreased compared with that of wild-type cells. We also demonstrated that WNV particles accumulated in the recycling endosomes in WNV-infected cells. In summary, these results suggest that Rab8b is involved in trafficking of WNV particles from recycling endosomes to the plasma membrane.