LC-MS/MS based comparative proteomics of floral nectars reveal different mechanisms involved in floral defense of Nicotiana spp., Petunia hybrida and Datura stramonium
LC-MS/MS based comparative proteomics of floral nectars reveal different mechanisms involved in floral defense of Nicotiana spp., Petunia hybrida and Datura stramonium
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DOI：
10.1016/j.jprot.2019.103618
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发表时间：
2020-02-20
影响因子：
3.3
通讯作者：
Thornburg, Robert
中科院分区：
文献类型：
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作者：
Silva, Fredy A.;Guirgis, Adel;Thornburg, Robert
烟草花蜜（FN）是由糖、氨基酸和称为花蜜的蛋白质组成的蜜腺产生的一种生物液体，参与花的防御。FN为微生物提供了理想的营养来源。了解花蜜蛋白的作用对预测微生物生长、组成和植物-传粉者相互作用的影响至关重要。采用LC-MS/MS-based比较蛋白质组学分析方法，鉴定了22个来自P. hybrida的蛋白，35个来自D. stramonium的蛋白，以及144个来自Nicotiana的蛋白。数据可在proteomexchange （PXD014760）上获得。氧化石墨烯分析和分泌信号预测表明，防御/应激是烟草属中最大的蛋白质群。烟草蛋白组由105种蛋白质组成，包括脂质转移蛋白（LTPs）、花蜜氧化还原循环蛋白、蛋白酶抑制剂和pr蛋白。分类切片分析表明，ltp在波曲科和夜花科中含量最多，而油桃在Rusticae、Suaveolens、Polydicliae和Alata等科中含量最多。过氧化物酶（Pox）和几丁质酶（Chit）是杂交稻所独有的，而麦秆草只有7种独特的蛋白质。生化分析证实了这些差异。这些发现支持了一种假设，即尽管保守，但与防御/应激相关的蛋白质丰度差异可能会影响花的防御机制。意义：本研究代表了烟草属花花蜜与两个相关的茄属植物的比较蛋白质组学分析。研究发现，不同分类片段的蛋白质组之间存在显著差异，这为花蜜氧化还原循环、抗菌蛋白和信号通路的防御/应激相关蛋白组提供了相关的见解。FNs蛋白的活性影响微生物的生长。这些蛋白质组的知识为了解花蜜中分泌的蛋白质的多样性以及烟草属植物在其花卉防御中使用的一系列机制提供了重要的见解。
Tobacco floral nectar (FN) is a biological fluid produced by nectaries composed of sugars, amino acids and proteins called nectarins, involved in the floral defense. FN provides an ideal source of nutrients for microorganisms. Understanding the role of nectar proteins is essential to predict impacts in microbial growth, composition and plants-pollinators interactions. Using LC-MS/MS-based comparative proteomic analysis we identified 22 proteins from P. hybrida, 35 proteins from D. stramonium, and 144 proteins from 23 species of Nicotiana. The data are available at ProteomeXchance (PXD014760). GO analysis and secretory signal prediction demonstrated that defense/stress was the largest group of proteins in the genus Nicotiana. The Nicotiana spp. proteome consisted of 105 exclusive proteins such as lipid transfer proteins (LTPs), Nectar Redox Cycle proteins, proteases inhibitors, and PR-proteins. Analysis by taxonomic sections demonstrated that LTPs were most abundant in Undulatae and Noctiflora, while nectarins were more abundant in Rusticae, Suaveolens, Polydicliae, and Alata sections. Peroxidases (Pox) and chitinases (Chit) were exclusive to P. hybrida, while D. stramonium had only seven unique proteins. Biochemical analysis confirmed these differences. These findings support the hypothesis that, although conserved, there is differential abundance of proteins related to defense/stress which may impact the mechanisms of floral defense.Significance: This study represents a comparative proteomic analysis of floral nectars of the Nicotiana spp. with two correlated Solanaceous species. Significant differences were identified between the proteome of taxonomic sections providing relevant insights into the group of proteins related to defense/stress associated with Nectar Redox Cycle, antimicrobial proteins and signaling pathways. The activity of FNs proteins is suggested impact the microbial growth. The knowledge about these proteomes provides significant insights into the diversity of proteins secreted in the nectars and the array of mechanisms used by Nicotiana spp. in its floral defense.