Localisation of iron and zinc in grain of biofortified wheat
Localisation of iron and zinc in grain of biofortified wheat
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DOI：
10.1016/j.jcs.2022.103470
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发表时间：
2022-04-07
影响因子：
3.8
通讯作者：
Shewry, Peter R.
中科院分区：
文献类型：
--
作者：
Wan, Yongfang;Stewart, Theodora;Shewry, Peter R.
谷物中铁、锌的膳食贡献由含量、部位和化学形态决定。一个基因生物强化小麦线显示出更高的浓度的锌和铁比三个控制线时，超过两年的增长。使用成像（组织化学染色和LA-ICP-MS），连续珍珠和手解剖确定的矿物质分布显示两条线之间没有一致的差异。Fe在糊粉层和盾片中含量最高，Zn在盾片上皮、胚乳传递细胞和胚轴中含量最高。除最外层外，各粒级中P、Zn、Fe含量均呈正相关。这与铁和锌集中在植酸盐中是一致的。发育中的颗粒显示出从近端到远端的浓度梯度降低。因此，铁和锌的浓度较高的生物强化线比对照线，但它们的位置没有差异。
The dietary contributions of iron (Fe) and zinc (Zn) from cereals are determined by concentrations, locations and chemical forms. A genetically biofortified wheat line showed higher concentrations of Zn and Fe than three control lines when grown over two years. The mineral distributions determined using imaging (histochemical staining and LA-ICP-MS), sequential pearling and hand dissection showed no consistent differences between the two lines. Fe was most abundant in the aleurone layer and the scutellum and Zn in the scutellar epithelium, the endosperm transfer cells and embryonic axis. Pearling fractions showed positive correlations between the concentration of P and those of Zn and Fe in all fractions except the outermost layer. This is consistent with Fe and Zn being concentrated in phytates. Developing grains showed decreasing gradients in concentration from the proximal to the distal ends. The concentrations of Fe and Zn were therefore higher in the biofortified line than the control lines but their locations did not differ.