Experimental phage therapy against Staphylococcus aureus in mice
Experimental phage therapy against Staphylococcus aureus in mice
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DOI：
10.1128/aac.01513-06
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发表时间：
2007-08-01
影响因子：
4.9
通讯作者：
Iannelli, Domenico
中科院分区：
文献类型：
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作者：
Capparelli, Rosanna;Parlato, Marianna;Iannelli, Domenico
本研究描述了一种对金黄色葡萄球菌（包括耐甲氧西林葡萄球菌菌株）具有活性的噬菌体（M-Sa）。当与金黄色葡萄球菌A170（10（8）CFU/小鼠）同时接种小鼠时，噬菌体（10（9）PFU）拯救了97％的小鼠；当应用于非致死（5 X 10(6) CFU/小鼠）10天感染时，噬菌体也完全清除了细菌。由金黄色葡萄球菌递送至巨噬细胞内的噬菌体 M-Sa 可在体内和体外杀死细胞内葡萄球菌。噬菌体还可以防止脓肿形成并减少脓肿的细菌负荷和重量。这些结果表明噬菌体可能用于控制局部和全身人类金黄色葡萄球菌感染。
The present study describes a bacteriophage (M-Sa) active against Staphylococcus aureus, including methicillin-resistant staphylococcal strains. When inoculated into mice simultaneously with S. aureus A170 (10(8) CFU/mouse), phage (10(9) PFU) rescued 97% of the mice; when applied to nonlethal (5 X 10(6) CFU/mouse) 10-day infections, the phage also fully cleared the bacteria. The phage M-Sa, delivered inside macrophages by S. aureus, kills the intracellular staphylococci in vivo and in vitro. The phage can also prevent abscess formation and reduce the bacterial load and weight of abscesses. These results suggest a potential use of the phage for the control of both local and systemic human S. aureus infections.