Screening for polar lipids, antioxidant, and anti-inflammatory activities ofGloeothecesp. lipid extracts pursuing new phytochemicals from cyanobacteria
Screening for polar lipids, antioxidant, and anti-inflammatory activities ofGloeothecesp. lipid extracts pursuing new phytochemicals from cyanobacteria
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DOI：
10.1007/s10811-020-02173-6
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发表时间：
2020-06-26
影响因子：
3.3
通讯作者：
Domingues, M. Rosario
中科院分区：
文献类型：
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作者：
da Costa, Elisabete;Amaro, Helena M.;Domingues, M. Rosario
蓝细菌是光合生物，其产生具有有益特性的各种分子，用于诸如食品和饲料、药物和工业化学品等领域。特别是，来自藻类的极性脂质正在引起人们的兴趣，因为它们与生物活性特性和健康益处有关。蓝细菌的研究很少，特别是在粘囊藻的情况下。其极性脂质组未知。我们已经使用了高分辨率液相色谱-质谱法为基础的方法来确定极性脂质体的粘帚霉。并且还评估了它们的抗氧化和抗炎潜力。所获得的结果允许识别162个脂质分子种类的糖脂，甜菜碱脂质，磷脂类。将这些分子物质酯化成具有C16和C18链长且不饱和度（DoU）在0和4之间的脂肪酸（FA）。在脂质组中鉴定的一些脂质种类因其生物活性特性而被认可。因此，使用考克斯-2酶活性测定试剂盒评价极性脂质提取物对超氧化物（O-2（中心点-））和一氧化氮（（NO）-N-中心点）自由基的抗氧化能力以及抗炎能力。脂质提取物对O（2）（中心点-）和（中心点）NO自由基的抗氧化能力呈剂量依赖性，IC（50）分别为939.8 +/- 15.6和1033.7 +/- 17.9 μ g mL（-1）。该提取物通过抑制考克斯-2而抑制花生四烯酸转化为前列腺素H2（PGH 2），从而显示出抗炎活性，其中测试的最低浓度为10 μ g mL（-1），抑制PGH 2的产生约58%。总的来说，这些结果表明，从Gloeothece提取物可能是一种天然产物的来源，促进越来越多的兴趣蓝藻开发生产食品和代谢产物具有潜在的生物活性特性，可用于营养品，化妆品和医药应用。
Cyanobacteria are photosynthetic organisms that produce a variety of molecules with beneficial properties for applications in fields such as food and feed, pharmaceuticals, and industrial chemicals. In particular, polar lipids from algae are gaining interest because they have been associated with bioactive properties and health benefits. Cyanobacteria have been little studied, particularly in the case ofGloeothecesp. whose polar lipidome is unknown. We have used a high-resolution liquid chromatography mass spectrometry-based approach to identify the polar lipidome ofGloeothecesp. and have also evaluated their antioxidant and anti-inflammatory potential. Results obtained allowed the identification of 162 lipid molecular species from glycolipids, betaine lipids, and phospholipid categories. These molecular species were esterified to fatty acids (FA) with C16 and C18 chain length and with a degree of unsaturation (DoU) ranging between 0 and 4. Some of the lipid species identified in the lipidome are recognized for their bioactive properties. Therefore, the antioxidant capacity of the polar lipid extracts was evaluated against superoxide (O-2(center dot-)) and nitric oxide ((NO)-N-center dot) radicals and the anti-inflammatory capacity using the COX-2 Enzyme Activity Assay Kit. The lipid extracts displayed antioxidant capacity toward O(2)(center dot-)and(center dot)NO radicals in a dose-dependent manner with IC(50)of 939.8 +/- 15.6 and 1033.7 +/- 17.9 mu g mL(-1), respectively. The extracts showed anti-inflammatory activity by the inhibition of the conversion of arachidonic acid to prostaglandin H2 (PGH2) via inhibition of COX-2, with the lowest concentration tested of 10 mu g mL(-1)inhibiting the production of PGH2 by about 58%. Overall, these results suggest that the extracts fromGloeothececould be a source of natural product, fostering increasing interest of cyanobacterial exploitation to produce food and metabolites with potential bioactive properties useful for nutraceutical, cosmetics, and medicinal applications.