Polypeptides immunologically related to band 3 are present in nucleated somatic cells.
Polypeptides immunologically related to band 3 are present in nucleated somatic cells.
复制标题
与带3免疫学相关的多肽存在于有核体细胞中。
DOI：
10.1073/pnas.80.22.6882
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发表时间：
1983
影响因子：
11.1
通讯作者：
P. S. Bassel
中科院分区：
文献类型：
--
作者：
M. Kay;C. Tracey;J. Goodman;J. Cone;P. S. Bassel
带3是红细胞的主要跨膜多肽，介导阴离子（氯离子和碳酸氢根）跨膜交换。我们怀疑带3存在于有核体细胞以及红细胞上，因为与带3免疫相关的衰老细胞抗原存在于淋巴细胞、血小板、成体肝细胞和胚胎肾细胞上;并且针对从白细胞分离的衰老细胞抗原制备的抗体与红细胞带3反应。出于这个原因，我们研究了人类成纤维细胞，肺细胞，中性粒细胞，单核白细胞，鳞状上皮（口）细胞，肺鳞状上皮癌，小鼠神经母细胞瘤细胞，和大鼠肝细胞带3的免疫反应形式，通过使用单特异性抗体红细胞带3。结果表明，共享共同的抗原决定簇与红细胞带3的多肽是存在于有核体细胞的免疫荧光，免疫电子显微镜，和免疫放射自显影确定。肽图谱显示红细胞带3和白细胞带3样蛋白之间的序列同源性。免疫荧光研究表明，有核细胞中的带3样蛋白参与抗体诱导的细胞表面加帽。
Band 3, the major transmembrane polypeptide of erythrocytes, mediates the exchange of anions (chloride and bicarbonate) across the membrane. We suspected that band 3 was present on nucleated somatic cells as well as erythrocytes because the senescent cell antigen that is immunologically related to band 3 is present on lymphocytes, platelets, adult liver cells, and embryonic kidney cells; and antibodies prepared against the senescent cell antigen isolated from leukocytes react with erythrocyte band 3. For this reason, we examined human fibroblasts, lung cells, neutrophils, mononuclear leukocytes, squamous epithelial (mouth) cells, lung squamous epithelial carcinoma, mouse neuroblastoma cells, and rat hepatocytes for immunoreactive forms of band 3 by using monospecific antibodies to erythrocyte band 3. The results demonstrated that polypeptides sharing common antigenic determinants with erythrocyte band 3 are present in nucleated somatic cells as determined by immunofluorescence, immunoelectron microscopy, and immunoautoradiography. Peptide mapping revealed substantial sequence homology between erythrocyte band 3 and the band 3-like protein of leukocytes. Immunofluorescence studies indicate that the band 3-like proteins in nucleated cells participate in antibody-induced cell surface capping.