Use of Capillary Zone Electrophoresis for the Analysis of DNA-BINDING to a Peptide Derived from Amyloid P Component
Use of Capillary Zone Electrophoresis for the Analysis of DNA-BINDING to a Peptide Derived from Amyloid P Component
复制标题
使用毛细管区带电泳分析淀粉样蛋白 P 成分衍生肽的 DNA 结合
DOI：
10.1080/10826079308019906
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发表时间：
1993
影响因子：
1.3
通讯作者：
F. Robey
中科院分区：
文献类型：
--
作者：
N. Heegaard;F. Robey
摘要 使用毛细管区带电泳来表征寡核苷酸与源自人血清淀粉样蛋白 P 成分的合成肽之间的结合相互作用。通过电泳从含有游离和肽结合寡核苷酸的溶液中将游离核苷酸与复合物分离。通过这种方式，可以使用微量未标记的样品快速评估结合的定性和定量方面。可以表征核苷酸和肽结合的最小结构和序列特异性，并且基于电泳分析的定量输出，估计结合常数。理论上，该方法适用于任何分子相互作用，其中复合物的电荷/质量比不同于游离分子，并且其中至少一种相互作用的组分在毛细管电泳系统中是可定量回收和检测的。因此，它是唯一可用于简单快速的方法...
Abstract Capillary zone electrophoresis was used to characterize the binding interactions between oligonucleotides and synthetic peptides derived from human serum amyloid P component. From a solution containing free and peptide-bound oligonucleotides, free nucleotide was separated from the complex by means of electrophoresis. In this way, both qualitative and quantitative aspects of the binding could be assessed rapidly using minute amounts of unlabelled samples. Minimal structures and sequence specificity of binding of nucleotides and peptides could be characterized and, based on the quantitative output of the electrophoretic analysis, binding constants were estimated. In theory, the approach is applicable for any molecular interaction where the charge/mass ratio of complexes differ from the free molecules and where at least one of the interacting components is quantitatively recoverable and detectable in the capillary electrophoresis system. As such, it is the only method available for simple and fast e...