Dual regulation by phospho-OmpR of ssrA/B gene expression in Salmonella pathogenicity island 2
Dual regulation by phospho-OmpR of ssrA/B gene expression in Salmonella pathogenicity island 2
复制标题
DOI：
10.1046/j.1365-2958.2003.03502.x
复制
发表时间：
2003-05-01
影响因子：
3.6
通讯作者：
Kenney, LJ
中科院分区：
文献类型：
--
作者：
Feng, XH;Oropeza, R;Kenney, LJ
沙门氏菌致病岛2（SPI-2）上的基因表达是小鼠全身感染所必需的。该区域编码III型分泌系统、分泌效应物和双组分调节系统SsrA/B（也称为SpiR），以及另外的未表征的基因。在目前的工作中，我们证明了磷酸化OmpR（OmpR-P）的功能作为spiC-ssrA/B位点的激活剂。在spiR处有两个启动子;一个在ssrA的上游，另一个在ssrB的上游。我们的研究结果表明，在许多两个组件的监管系统相比，传感器激酶SsrA的调节似乎是解耦的，并从响应调节器SsrB的调节不同。ssrA/B的OmpR调节是已知的仅有的几个例子之一，其中双组分反应调节剂直接调节另一个双组分调节系统的表达。
Expression of genes located on Salmonella pathogenicity island 2 (SPI-2) is required for systemic infection in mice. This region encodes a type III secretion system, secreted effectors and the two-component regulatory system SsrA/B (also referred to as SpiR), as well as additional uncharacterized genes. In the present work, we demonstrate that phospho-OmpR (OmpR-P) functions as an activator at the spiC-ssrA/B locus. There are two promoters at spiR ; one is upstream of ssrA and the other upstream of ssrB . Our results indicate that, in contrast to many two-component regulatory systems, regulation of the sensor kinase SsrA appears to be uncoupled and distinct from regulation of the response regulator SsrB. OmpR regulation of ssrA/B is one of only a few examples known in which a two-component response regulator directly regulates the expression of another two-component regulatory system.